ISS014-E-17547 (20 March 2007) --- Astronaut Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer, prepares to insert a test sample in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.