jsc2025e007249 (10/29/2024) --- A preflight image of sixteen BioNutrients-3 yogurt bags attached to the Space Automated Bioproduct Laboratory (SABL) Tray mockup during the BioNutrients-3 Experiment Verification Test. Below the bags, the purple and pink SABL interface board, which is used for pH comparison, is visible. The pink color of the bags indicates that these yogurt bags have completed their incubation, causing the pH indicator to change from purple to pink. Image courtesy of NASA Ames. BioNutrients-3 builds on results from the BioNutrients and BioNutrients-2 investigations, seeking to develop a method to produce on-demand nutrients and supplement food stores. Producing vitamins and other nutrients in-flight could help maintain the health of crew members on long-duration missions while reducing launch mass and volume. BioNutrients-3 incorporates new food safety features as well as bioengineered yeast and bacteria.