
The Science Verification Test for NASA’s Advanced Plant Experiment-08 (APEX-08) testing Arabidopsis thaliana, a plant scientists routinely use for research, takes place inside the Veggie growth chamber at NASA’s Kennedy Space Center in Florida on Nov. 6, 2020. The test is part of the process for demonstrating readiness for space research ahead of its flight on SpaceX’s 23rd Commercial Resupply Services mission to the International Space Station. The APEX-08 study includes making genetic alterations that elicit a response in a group of organic compounds that modulate plant responses to environmental stress.

The Science Verification Test for NASA’s Advanced Plant Experiment-08 (APEX-08) testing Arabidopsis thaliana, a plant scientists routinely use for research, takes place inside the Veggie growth chamber at NASA’s Kennedy Space Center in Florida on Nov. 6, 2020. The test is part of the process for demonstrating readiness for space research ahead of its flight on SpaceX’s 23rd Commercial Resupply Services mission to the International Space Station. The APEX-08 study includes making genetic alterations that elicit a response in a group of organic compounds that modulate plant responses to environmental stress.

iss052e079049 (Aug. 31, 2017) --- NASA astronaut Jack Fischer with four cansiters of the Biological Research in Canisters-22 (BRIC-22) experiment in the Japanese Experiment Module (JEM) Pressurized Module (JPM). BRIC-22 studies the stress response in plants by comparing 8 different variants of thale cress (Arabidopsis thaliana) in the microgravity environment.

Jeffrey Richards, project science coordinator and research scientist at NASA’s Kennedy Space Center in Florida, demonstrates how Arabidopsis thaliana plant samples are tested in a 2D microgravity simulator in the Microgravity Simulation Support Facility on Dec. 20, 2018.
jsc2024e066519 (10/7/2024) --- The Comets set up the test tubes for the final trial of their experiment, Effects of Microgravity on Arabidopsis thaliana Seed Germination. Their experiment is part of the Nanoracks-National Center for Earth and Space Science Education-Surveyor-Student Spaceflight Experiments Program Mission 18 to ISS (Nanoracks-NCESSE-Surveyor-SSEP).

iss057e131531 (12/11/2018) --- A view of the APEX-05 Petri Plate in the Veggie facility onboard the International Space Station (ISS). The Spaceflight-induced Hypoxic/ROS Signaling (APEX-05) experiment grows different wild and mutant varieties of Arabidopsis thaliana, in order to understand how their genetic and molecular stress response systems work in space.

iss057e135002 (12/18/2018) --- Canadian Space Agency (CSA) astronaut David Saint-Jacques removes the APEX-05 Petri Plate from the FIR/LMM (Fluids Integrated Rack/Light Microscopy Module). The Spaceflight-induced Hypoxic/ROS Signaling (APEX-05) experiment grows different wild and mutant varieties of Arabidopsis thaliana, in order to understand how their genetic and molecular stress response systems work in space.
jsc2024e067100 (1/22/2024) --- The San Jacinto College – South Campus student team prepares a trial run to optimize germination conditions for their experiment, Comparison of Arabidopsis thaliana Germination and Cell Wall Growth in Microgravity versus Standard Conditions. Their experiment is part of the Nanoracks-National Center for Earth and Space Science Education-Surveyor-Student Spaceflight Experiments Program Mission 18 to ISS (Nanoracks-NCESSE-Surveyor-SSEP).

iss052e079058 (Aug. 31, 2017) --- NASA astronaut Jack Fischer actuating four cansiters of the Biological Research in Canisters-22 (BRIC-22) experiment in the Japanese Experiment Module (JEM) Pressurized Module (JPM). BRIC-22 studies the stress response in plants by comparing 8 different variants of thale cress (Arabidopsis thaliana) in the microgravity environment.

iss052e002352 (6/12/2017) --- A view of NASA astronaut Jack Fischer replacing the European Modular Cultivation System (EMCS) Experiment Containers (ECs) with new ECs prepared for the Seedling Growth 3 experiment. Seedling Growth-3 is the third part of the Seedling Growth Experiment series, using the plant Arabidopsis thaliana to investigate the effects of gravity on the cellular signaling mechanisms of light sensing in plants (phototropism), and to investigate cell growth and proliferation responses to light stimulation under microgravity conditions.

iss052e079043 (Aug. 31, 2017) --- NASA astronaut Jack Fischer actuating four cansiters of the Biological Research in Canisters-22 (BRIC-22) experiment in the Japanese Experiment Module (JEM) Pressurized Module (JPM). BRIC-22 studies the stress response in plants by comparing 8 different variants of thale cress (Arabidopsis thaliana) in the microgravity environment.

Arabidopsis thaliana plants are seen inside the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1 prior to harvest of half the plants. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in NASA Kennedy Space Center's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, harvests half the Arabidopsis thaliana plants inside the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

jsc2021e036657 (8/11/2021) --- Arabidopsis thaliana seedlings following 9 days of growth on 1.2% agar-based media in petri dishes in the VEGGIE growth chamber under temperature, humidity and CO2 conditions mimicking those recorded at the International Space Station. The genotypes of these plants are indicated at the bottom of each panel. These images were taken during the Science Verification Test carried out at NASA Kennedy Space Center. All genotypes grew equally well under these conditions except for the cuao3-100cdr7 mutant, which grew more slowly.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, uses a FluorPen to measure the chlorophyll fluorescence of Arabidopsis thaliana plants inside the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1. Half the plants were then harvested. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Team members pause for a photo after the successful harvest of half the Arabidopsis thaliana plants inside the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1. From right to left are Jeff Richards with Stinger-Ghaffarian Technologies; David Hanson, part of the principal investigator's team; Oscar Monje with NASA Kennedy Space Center's Engineering Services Contract; and John "JC" Carver, a payload integration engineer with Kennedy's Test and Operations Support Contract. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, places Arabidopsis thaliana plants harvested from the Advanced Plant Habitat (APH) Flight Unit No. 1 into an Ultra-low Freezer chilled to -150 degrees Celsius. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, places Arabidopsis thaliana plants harvested from the Advanced Plant Habitat (APH) Flight Unit No. 1 into a Mini ColdBag that quickly freezes the plants. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, opens the door to the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1 for a test harvest of half of the Arabidopsis thaliana plants growing within. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, uses a FluorPen to measure the chlorophyll fluorescence of Arabidopsis thaliana plants inside the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1. Half the plants were then harvested. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, opens the door to the growth chamber of the Advanced Plant Habitat (APH) Flight Unit No. 1 for a test harvest of half of the Arabidopsis thaliana plants growing within. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.