Commander Jack Lousma works with Electrophoresis Equipment Verification Test (EEVT) electrophoresis unit, cryogenic freezer and tube, and stowage locker equipment located on crew compartment middeck aft bulkhead.

41D-06-013 (6 Sept 1984) --- Payload specialist Charles Walker works with the continuous flow electrophoresis systems (CFES) experiment, located in the middeck.

61B-02-014 (26 Nov-3 Dec 1985) --- Payload Specialist Charles D. Walker works with the handheld protein growth experiment -- one of a series of tests being flown to study the possibility of crystallizing biological materials. Walker rests the experiment against the larger continuous flow electrophoresis systems experiment.

S84-35757 (May 1984) --- Astronaut Judith A. Resnik, 41-D mission specialist, and Charles Walker, payload specialist for that June 1984 flight, prepare for some scheduled intravehicular activity involving the continuous flow electrophoresis systems (CFES) experiment. CFES will join the six-member crew aboard the Earth-orbiting Discovery for a seven day mission. The two share in preparing a sample to be processed by the CFES. In the background are stowage lockers and a CFES trainer-- part of the Shuttle one-g trainer at NASA's Johnson Space Center (JSC). Walker, an engineer at McDonnell Douglas Astronautics Co. in St. Louis, Missouri, will be the first Shuttle payload specialist to represent a project designed for commercial purposes. As payload specialist, his job will be to run the materials electrophoresis-operations-in-space project. The project is aimed at separating large quantities of biological materials in space for ultimate use in new pharmaceuticals. The photo was taken by a McDonnell Douglas photographer.

STS047-03-024 (12 - 20 Sept 1992) --- Astronaut N. Jan Davis, mission specialist, talks to ground controllers as she works with the Free Flow Electrophoresis Unit (FFEU) in the Science Module of the Earth-orbiting Space Shuttle Endeavour. Davis joined five other NASA astronauts and a Japanese payload specialist for eight days of scientific research onboard Endeavour.

STS007-11-495 (18-24 June 1983) --- Astronaut Sally K. Ride, STS-7 mission specialist, communicates with ground controllers from the mid deck of the Earth-orbiting Space Shuttle Challenger. She has just opened one of the large lockers during the operation and monitoring of the continuous flow electrophoresis system (CFES) experiment at left edge. This photograph was made with a 35mm camera.

KSC-84PC-248 (For release Aug. 27, 1984) --- The Continuous Flow Electrophoresis System (CFES) is being installed in the middeck of the Orbiter Discovery in preparation for the flight of mission STS-41D in June. The CFES, originating from the McDonnell Douglas Astronautics Co. includes a fluid systems module, and experiment control and monitoring module, a sample storage module and a pump/accumulator package along with miscellaneous equipment stored in a middeck locker. Photo credit: NASA

STS006-03-381 (4-9 April 1983) --- Astronaut F. Story Musgrave, STS-6 mission specialist, monitors the activity of a sample in the continuous flow electrophoresis system (CFES) aboard the Earth-orbiting space shuttle Challenger. Dr. Musgrave is in the middeck area of the spacecraft. He has mounted a 35mm camera to record the activity through the window of the experiment. This frame was also photographed with a 35mm camera. Photo credit: NASA

STS007-02-020 (21 June 1983) --- Astronaut Sally K. Ride, STS-7 mission specialist, STS-7 mission specialist, stands in the mid deck of the orbiting Space Shuttle Challenger near one of the experiment with which she has devoted a great deal of time. The continuous flow electrophoresis system (CFES) experiment, about the size of a household refrigerator, stands nearby. One of her fellow crewmembers moves partially out of frame in the background. The tube on her face is part of a communications system linking Dr. Ride to ground controllers in Houston.

These gels were obtained by two-dimensional (2D) electrophoresis, in which proteins move different substances through a polyacrylamide gel matrix based on their molecular weight and total charge in an electric field. The gels illustrate principal investigator David Niesel’s findings that exposure to modeled microgravity results in some Streptoccoccus Pneumonia’s proteins being upregulated and others being downregulated. In 2D protein profiles of whole cell lysates of Streptoccoccus Pneumonia, 6,304 cultured under normal gravity (left), appear to be expressed at higher levels indicated with black circles. Red circles (right) indicate proteins that were grown under modeled microgravity in a high aspect ratio vessel HARV).

STS004-28-312 (27 June-4 July 1982) --- Astronaut Thomas K. Mattingly II, STS-4 crew commander, prepares a meal in the middeck area of space shuttle Columbia. He uses scissors to open a drink container. Various packages of food and meal accessories are attached to locker doors. At far left edge of the frame is the tall payload called continuous flow electrophoresis experiment (CFES) system-designed to separate biological materials according to their surface electrical charges as they pass through an electrical field. Astronaut Henry W. Hartsfield Jr. exposed this frame with a 35mm camera. Photo credit: NASA

STS007-08-396 (18-24 June 1983) --- Astronaut Robert L. Crippen, STS-7 commander, used a 35mm camera to expose this frame of the four representatives of the "thirty five new guys" onboard the Earth-orbiting Space Shuttle Challenger. The TFNG (notice Frederick H. Hauck's T-shirt at left) pays tribute to the 1978 astronaut candidate (ASCAN) class of NASA astronauts. Others in the photograph are Astronauts Norman E. Thagard, Sally K. Ride and John M. Fabian. Hauck is the crew's pilot and the other three are mission specialist. The crew members are in the process of preparing a snack of meal. The tall experiment in the background is the Continuous Flow Electrophoresis System (CFES).

S86-41700 (19 May 1984) --- The Space Shuttle Discovery moves towards Pad A on the crawler transporter for its maiden flight. Discovery will be launched on its first mission no earlier than June 19, 1984. Flight 41-D will carry a crew of six; Commander Henry Hartsfield, Pilot Mike Coats, Mission Specialists Dr. Judith Resnik, Dr. Steven Hawley and Richard Mullane and Payload Specialist Charles Walker. Walker is the first payload specialist to fly aboard a space shuttle. He will be running the materials processing device developed by McDonnell Douglas as part of its Electrophoresis Operations in Space project. Mission 41-D is scheduled to be a seven-day flight and to land at Edwards Air Force Base in California. The Syncom IV-1 (LEASAT) will be deployed from Discovery's cargo bay and the OAST-1, Large Format Camera, IMAX and Cinema 360 cameras will be aboard.

S82-28911 (March 1982) --- The L-shaped experiment in the right half of this photo was one of a number of scientific experiments which made the trip for NASA's third space transportation system (STS-3) mission, along with astronauts Jack R. Lousma, pictured, and C. Gordon Fullerton. The experiment, making encore in space (it also flew on the Apollo Soyuz Test Project in 1985), is designed to evaluate the feasibility of separating cells according to their surface electrical charge. It is a forerunner to planned experiments with other equipment that will purify biological materials in the low gravity environment of space. The process of electrophoresis utilizes an electric field to separate cells, and other biological material in fluids without damaging the cells which can then be used in the study of cell biology, immunology and medical research. This photograph was taken with a 35mm camera by Fullerton. Photo credit: NASA

41D-12-034 (30 Aug.- 5 Sept. 1984) --- Following the completion of their six-day mission in space, the six crew members of NASA's 41-D mission mentioned that though a great deal of work was accomplished, there were "fun" moments too. From all appearance this group shot was one of the lighter moments aboard the Discovery. Crew members are (counter-clockwise from center) Henry W. Hartsfield Jr., crew commander; Michael L. Coats, pilot; Steven A. Hawley and Judith A. Resnik, both mission specialists; Charles D. Walker, payload specialist; and Richard M. (Mike) Mullane, mission specialist. A pre-set 35mm camera was used to expose the frame. Walker stands near the project that occupied the majority of his time onboard--the continuous flow electrophoresis systems (CFES) experiment. Photo credit: NASA