KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians position the the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) science rack for installation into the Multi-Purpose Logisitics Module Leonardo. Leonardo will fly on Space Shuttle Atlantis on mission STS-121. The MELFI will provide cooling and storage for scientific experiment samples and perishable materials in four insulated containers, known as dewars, with independently selectable temperatures of -80 degrees Celsius, -26 degrees Celsius, and +4 degrees Celsius. MELFI will also be used to transport samples to and from the Station. MELFI is provided as laboratory support equipment by the European Space Agency. STS-121 is the second Return to Flight mission to the International Space Station. The launch window extends from Sept. 9 through Sept. 24.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians install the the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) science rack into the Multi-Purpose Logisitics Module Leonardo. Leonardo will fly on Space Shuttle Atlantis on mission STS-121. The MELFI will provide cooling and storage for scientific experiment samples and perishable materials in four insulated containers, known as dewars, with independently selectable temperatures of -80 degrees Celsius, -26 degrees Celsius, and +4 degrees Celsius. MELFI will also be used to transport samples to and from the Station. MELFI is provided as laboratory support equipment by the European Space Agency. STS-121 is the second Return to Flight mission to the International Space Station. The launch window extends from Sept. 9 through Sept. 24.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) science rack (left) is moved into position for installation into the Multi-Purpose Logisitics Module Leonardo. Leonardo will fly on Space Shuttle Atlantis on mission STS-121. The MELFI will provide cooling and storage for scientific experiment samples and perishable materials in four insulated containers, known as dewars, with independently selectable temperatures of -80 degrees Celsius, -26 degrees Celsius, and +4 degrees Celsius. MELFI will also be used to transport samples to and from the Station. MELFI is provided as laboratory support equipment by the European Space Agency. STS-121 is the second Return to Flight mission to the International Space Station. The launch window extends from Sept. 9 through Sept. 24.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians prepare to install the the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) science rack into the Multi-Purpose Logisitics Module Leonardo. Leonardo will fly on Space Shuttle Atlantis on mission STS-121. The MELFI will provide cooling and storage for scientific experiment samples and perishable materials in four insulated containers, known as dewars, with independently selectable temperatures of -80 degrees Celsius, -26 degrees Celsius, and +4 degrees Celsius. MELFI will also be used to transport samples to and from the Station. MELFI is provided as laboratory support equipment by the European Space Agency. STS-121 is the second Return to Flight mission to the International Space Station. The launch window extends from Sept. 9 through Sept. 24.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, a technician prepares to install the the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) science rack into the Multi-Purpose Logisitics Module Leonardo. Leonardo will fly on Space Shuttle Atlantis on mission STS-121. The MELFI will provide cooling and storage for scientific experiment samples and perishable materials in four insulated containers, known as dewars, with independently selectable temperatures of -80 degrees Celsius, -26 degrees Celsius, and +4 degrees Celsius. MELFI will also be used to transport samples to and from the Station. MELFI is provided as laboratory support equipment by the European Space Agency. STS-121 is the second Return to Flight mission to the International Space Station. The launch window extends from Sept. 9 through Sept. 24.

ISS025-E-006391 (8 Oct. 2010) --- NASA astronaut Shannon Walker, Expedition 25 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Astronaut Karen Nyberg,Expedition 36 flight engineer,works with samples in Minus Eighty-Degree Laboratory Freezer for ISS (MELFI-3) in the Destiny laboratory of the Earth-orbiting International Space Station.

ISS025-E-006389 (8 Oct. 2010) --- NASA astronaut Shannon Walker, Expedition 25 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS033-E-016196 (26 Oct. 2012) --- Japan Aerospace Exploration Agency astronaut Aki Hoshide, Expedition 33 flight engineer, closes a dewar cover after servicing the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS036-E-006564 (6 June 2013) --- European Space Agency astronaut Luca Parmitano, Expedition 36 flight engineer, works with samples in Minus Eighty-Degree Laboratory Freezer for ISS (MELFI-3) in the Destiny laboratory of the Earth-orbiting International Space Station.

ISS033-E-016183 (26 Oct. 2012) --- Japan Aerospace Exploration Agency astronaut Aki Hoshide, Expedition 33 flight engineer, services the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS043E207615 (05/18/2015) --- Expedition 43 Commander Terry Virts on the International Space Station works with experiment samples stored inside one of the station’s Minus Eighty-Degree Laboratory Freezer for ISS, or MELFI. These laboratory freezers are used to maintain experiment samples at ultra-cold temperatures throughout a mission.

ISS033-E-016181 (26 Oct. 2012) --- NASA astronaut Sunita Williams, Expedition 33 commander; and Japan Aerospace Exploration Agency astronaut Aki Hoshide, flight engineer, prepare to service the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

iss050e057444 (3/15/2017) --- NASA astronaut Shane Kimbrough removing a storage locker in the Minus Eighty-degree Laboratory Freezer for ISS (MELFI) to store samples from an experiment, in the U.S. Laboratory. The Minus Eighty-Degree Laboratory Freezer for ISS (MELFI) is a cold storage unit that maintains experiment samples at ultra-cold temperatures throughout a mission.

ISS026-E-012192 (26 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS026-E-012172 (26 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS024-E-006978 (27 June 2010) --- NASA astronaut Doug Wheelock, Expedition 24 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS026-E-012185 (26 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

iss050e011020 (11/21/2016) --- European Space Agency (ESA) astronaut Thomas Pesquet adding MARROW samples to the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Japanese Experiment Module (JEM) Pressurized Module (JPM). The MARROW study (Bone Marrow Adipose Reaction: Red Or White?) (Marrow) investigation looks at the effect of microgravity on the bone marrow.

ISS024-E-006697 (28 June 2010) --- NASA astronaut Doug Wheelock, Expedition 24 flight engineer, prepares to insert biological samples into trays in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS020-E-026348 (30 July 2009) --- Astronaut Tim Kopra, Expedition 20 flight engineer, smiles for the camera as he completes the task of placing samples taken for the Nutritional Status Assessment (NUTRITION) w/Repository study into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station.

ISS024-E-006707 (28 June 2010) --- After inserting biological samples, NASA astronaut Doug Wheelock, Expedition 24 flight engineer, replaces a dewar tray in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS024-E-006699 (28 June 2010) --- NASA astronaut Doug Wheelock, Expedition 24 flight engineer, prepares to insert biological samples into trays in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS024-E-007346 (2 July 2010) --- NASA astronauts Tracy Caldwell Dyson (background) and Shannon Walker, both Expedition 24 flight engineers, prepare to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS027-E-014286 (14 April 2011) --- NASA astronaut Ron Garan, Expedition 27 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

iss027e014283 (4/13/2011) --- Expedition 27 flight engineer Ron Garan prepares to stow a blood draw for the CSA (Canadian Space Agency) Vascular Blood Collection protocol in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS024-E-007331 (2 July 2010) --- After inserting biological samples, NASA astronaut Shannon Walker, Expedition 24 flight engineer, replaces a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS024-E-006705 (28 June 2010) --- After inserting biological samples, NASA astronaut Doug Wheelock, Expedition 24 flight engineer, replaces a dewar tray in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS034-E-067256 (12 March 2013) --- Expedition 34 Flight Engineer Tom Marshburn of NASA works with samples at the Minus Eighty-Degree Laboratory Freezer for the International Space Station (MELFI) in the U.S. lab Destiny aboard the International Space Station.

ISS024-E-007344 (2 July 2010) --- After inserting biological samples, NASA astronaut Shannon Walker, Expedition 24 flight engineer, replaces a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS023-E-030772 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS023-E-030773 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS025-E-005731 (1 Oct. 2010) --- NASA astronaut Shannon Walker, Expedition 25 flight engineer, services the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS023-E-030780 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS023-E-030771 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS024-E-012546 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS028-E-013799 (1 July 2011) --- NASA astronaut Mike Fossum, Expedition 28 flight engineer, replaces a failed electronic unit for the Minus Eighty Laboratory Freezer for ISS 3 (MELFI-3) with a spare unit in the Kibo laboratory of the International Space Station.

ISS030-E-116988 (27 Feb. 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, is pictured near the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS026-E-023814 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

ISS026-E-023768 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, prepares to insert samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

ISS020-E-007156 (7 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

ISS028-E-013797 (1 July 2011) --- NASA astronaut Mike Fossum, Expedition 28 flight engineer, replaces a failed electronic unit for the Minus Eighty Laboratory Freezer for ISS 3 (MELFI-3) with a spare unit in the Kibo laboratory of the International Space Station.

ISS024-E-012555 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS019-E-005715 (11 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

ISS024-E-012553 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS024-E-012548 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS020-E-010021 (15 June 2009) --- Canadian Space Agency astronaut Robert Thirsk (left) and Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, both Expedition 20 flight engineers, prepare to put samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

iss070e108265 (3/6/2024) --- Flight Engineer Jasmin Moghbeli works to retrieve Media Bags for the SS: Engineering Stem Cell-Derived Cardiac Microtissues with Metabolic Regulators in Space to Promote Cardiomyocyte Maturation (Project EAGLE) investigation from Minus Eighty Degree Laboratory Freezer for ISS-1 (MELFI-1). Photo was taken in the Kibo Japanese Experiment Module (JEM).

ISS034-E-067263 (12 March 2013) --- Canadian astronaut Chris Hadfield, right, assists fellow Expedition 34 flight engineer and NASA astronaut Tom Marshburn during Minus Eighty-Degree Laboratory Freezer for International Space Station (MELFI)operations. The two are doing transfers of samples connected to the General Laboratory Active Cryogenic ISS Experiment Refrigerator or GLACIER in the U.S. lab Destiny.

View of Astronaut Reid Wiseman,Expedition 40 flight engineer,inserting urine samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) located in the Japanese Experiment Module (JEM).

View of Astronaut Reid Wiseman,Expedition 40 flight engineer,inserting urine samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) located in the Japanese Experiment Module (JEM).

ISS020-E-010025 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

ISS020-E-010028 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, returns a dewar tray to the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

ISS034-E-023768 (7 Jan. 2013) --- Canadian Space Agency astronaut Chris Hadfield, Expedition 34 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS038-E-006765 (21 Nov. 2013) --- In the International Space Station's Destiny laboratory, NASA astronaut Rick Mastracchio, Expedition 38 flight engineer, prepares to replace a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays.

ISS030-E-116878 (9 Feb. 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS038-E-006757 (21 Nov. 2013) --- In the International Space Station's Destiny laboratory, NASA astronaut Rick Mastracchio, Expedition 38 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays.

ISS019-E-010165 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

iss029e028495 (10/18/2011) --- Japan Aerospace Exploration Agency astronaut Satoshi Furukawa,Expedition 29 flight engineer,prepares to put samples from the CSA (Canadian Space Agency) Vascular Blood Collection protocol into the MELFI-1 (Minus Eighty Laboratory Freezer for ISS 1) unit.

ISS034-E-023771 (7 Jan. 2013) --- Canadian Space Agency astronaut Chris Hadfield, Expedition 34 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS032-E-011639 (2 Aug. 2012) --- NASA astronaut Sunita Williams, Expedition 32 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS030-E-116879 (9 Feb. 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS034-E-026607 (7 Jan. 2013) --- NASA astronaut Tom Marshburn, Expedition 34 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

View of Canadian Space Agency (CSA) Chris Hadfield,Expedition 34 Flight Engineer (FE),preparing to insert biological samples in the Minus Eighty Laboratory Freezer for International Space Station (ISS) - (MELFI-1),in the Japanese Experiment Module (JEM) Pressurized Module (JPM). Photo was taken during Expedition 34.

ISS030-E-116886 (9 Feb. 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS019-E-010170 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

ISS030-E-050864 (26 Jan. 2012) --- NASA astronaut Don Pettit, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS021-E-012527 (26 Oct. 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 21 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station.

ISS030-E-033272 (24 Dec. 2011) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares to insert ESA Role of Apoptosis in Lymphocyte Depression 2 (ROALD-2) experiment samples into a Minus Eighty Laboratory Freezer for ISS (MELFI-1) dewar tray located in the International Space Station's Kibo laboratory.

ISS043e000724 (03/13/2015) --- ESA (European Space Agency) astronaut Samantha Cristoforetti, Expedition 43 flight engineer works daily on science and maintenance duties on board the International Space Station. She is inspecting the Minus Eighty-degree Laboratory Freezer called by the shorter title "MELFI-3 Cold Box inspection". Astronauts are trained for long periods at the Johnson Space Center and in Russia before their missions in space begin so that they are fully trained for these complex duties.

ISS017-E-017539 (27 Sept. 2008) --- NASA astronaut Greg Chamitoff, Expedition 17 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

ISS017-E-017541 (27 Sept. 2008) --- NASA astronaut Greg Chamitoff, Expedition 17 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

ISS015-E-10572 (1 June 2007) --- Astronaut Sunita L. Williams, Expedition 15 flight engineer, inserts test samples in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as a part of the Nutritional Status Assessment (Nutrition) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

ISS034-E-023786 (7 Jan. 2013) --- Canadian Space Agency astronaut Chris Hadfield, Expedition 34 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station. Russian cosmonaut Roman Romanenko, flight engineer, is visible near Hadfield.

iss050e057655 (3/15/2020) --- Photographic documentation during Auxin Transport sample transfer to Minus Eighty-Degree Laboratory Freezer for ISS (MELFI) insertion. Studies on Gravity-Controlled Growth and Development in Plants Using True Microgravity Conditions (Auxin Transport) clarifies the role of auxins in pea and maize (corn) seedlings grown in microgravity, leading to new insight into how gravity, or the lack of gravity, affects plant development.

ISS022-E-011880 (16 Dec. 2009) --- NASA astronaut Jeffrey Williams, Expedition 22 commander, services the Advanced Plant Experiments on Orbit-Cambium (APEX-C) payload in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS020-E-037820 (2 Sept. 2009) --- European Space Agency astronaut Christer Fuglesang (bottom) and NASA astronaut Tim Kopra, both STS-128 mission specialists, install a new Minus Eighty Degree Laboratory Freezer for ISS (MELFI) rack in the Destiny laboratory of the International Space Station.

ISS030-E-050849 (26 Jan. 2012) --- NASA astronaut Don Pettit, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS026-E-013561 (26 Dec. 2010) --- European Space Agency astronaut Paolo Nespoli, Expedition 26 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

S118-E-07456 (13 Aug. 2007) --- Astronaut Clay Anderson, Expedition 15 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Destiny laboratory of the International Space Station while Space Shuttle Endeavour (STS-118) remains docked with the station.

ISS022-E-015698 (28 Dec. 2009) --- Japan Aerospace Exploration Agency astronaut Soichi Noguchi (left) and NASA astronaut T.J. Creamer, both Expedition 22 flight engineers, service the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS022-E-011879 (16 Dec. 2009) --- NASA astronaut Jeffrey Williams, Expedition 22 commander, services the Advanced Plant Experiments on Orbit-Cambium (APEX-C) payload in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS022-E-011876 (16 Dec. 2009) --- NASA astronaut Jeffrey Williams, Expedition 22 commander, services the Advanced Plant Experiments on Orbit-Cambium (APEX-C) payload in the Minus Eighty Laboratory Freezer for ISS-2 (MELFI-2) in the Destiny laboratory of the International Space Station.

ISS026-E-013559 (26 Dec. 2010) --- European Space Agency astronaut Paolo Nespoli, Expedition 26 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS030-E-050848 (26 Jan. 2012) --- NASA astronaut Don Pettit, Expedition 30 flight engineer, prepares to insert biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

iss066e155896 (3/1/2022) --- DreamStar dolls float in front of Astrobee and Minus Eighty Laboratory Freezer for ISS (MELFI) aboard the International Space Station. The DreamStar science, technology, engineering, and mathematics (STEM) education project creates videos and educational materials highlighting women in space and reinforcing the message, “if you can see it, you can be it.”

iss066e155892 (3/1/2022) --- DreamStar dolls float in front of Astrobee and Minus Eighty Laboratory Freezer for ISS (MELFI) aboard the International Space Station. The DreamStar science, technology, engineering, and mathematics (STEM) education project creates videos and educational materials highlighting women in space and reinforcing the message, “if you can see it, you can be it.”

ISS028-E-014918 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

ISS028-E-014916 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

KENNEDY SPACE CENTER, FLA. - After removing its cover, technicians look over the Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

KENNEDY SPACE CENTER, FLA. - The Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station, is seen in the Space Station Processing Facility. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians remove the cover from the Minus Eighty Lab Freezer for ISS(MELFI) provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

iss050e057428 (03/15/2017) --- NASA astronaut Shane Kimbrough removes a storage locker in the Minus Eighty-degree Laboratory Freezer for ISS (MELFI) to store samples from an experiment. MELFI is a cold storage unit that maintains experiment samples at ultra-cold temperatures throughout a mission.

Photographic documentation of inspection and regular maintenance on Minus Eighty-degree Laboratory Freezer for the ISS 3 (MELFI-3) located in the LAB1S1 rack. View is of European Space Agency (ESA) astronaut Samantha Cristoforetti, Expedition 43 flight engineer, at work with the MELFI-3.

iss072e035693 (Oct. 11, 2024) --- NASA astronaut and Expedition 72 Flight Engineer Mike Barratt stows research samples in a science freezer, also known as the Minus Eighty-Degree Laboratory Freezer for ISS (MELFI). MELFI can preserve biological samples such as blood, microbes, plants, and more for retrieval and later analysis.

iss063e104373 (Oct. 7, 2020) --- Roscosmos cosmonaut and Expedition 63 Flight Engineer Ivan Vagner transfers biological samples into a science freezer for stowage and later analysis aboard the International Space Station.

ISS014-E-05124 (3 Oct. 2006) --- Astronaut Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer, works with the Passive Observatories for Experimental Microbial Systems in Micro-G (POEMS) payload in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

ISS015-E-35308 (19 Oct. 2007) --- NASA astronaut Peggy A. Whitson, Expedition 16 commander, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

ISS014-E-17550 (20 March 2007) --- Astronaut Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer, prepares to insert a test sample in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

ISS013-E-64637 (2 Aug. 2006) --- Astronaut Jeffrey N. Williams, Expedition 13 NASA space station science officer and flight engineer, works with the Passive Observatories for Experimental Microbial Systems in Micro-G (POEMS) payload in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.