61B-02-014 (26 Nov-3 Dec 1985) --- Payload Specialist Charles D. Walker works with the handheld protein growth experiment -- one of a series of tests being flown to study the possibility of crystallizing biological materials. Walker rests the experiment against the larger continuous flow electrophoresis systems experiment.
STS060-21-031 (3-11 Feb 1994) --- Using a lap top computer, astronaut N. Jan Davis monitors systems for the Commercial Protein Crystal Growth (CPCG) experiment onboard the Space Shuttle Discovery. Davis joined four other NASA astronauts and a Russian cosmonaut for eight days in space aboard Discovery.
iss062e087808 (3/11/2020) --- A view of Protein Crystal Growth-10 experiment hardware inside JAXA's (Japan Aerospace Exploration Agency) Kibo laboratory module aboard the International Space Station (ISS). Microgravity Crystallization of Glycogen Synthase-Glycogenin Protein Complex (CASIS PCG 10) crystallizes human glycogen synthase proteins on the space station. Determining the structure of the human glycogen synthase and full-length glycogenin protein complex could facilitate the development of treatments on Earth for metabolic disorders such as Type 2 diabetes, obesity, rare genetic disorders, and some forms of cancer.
STS072-310-007 (11-20 Jan. 1996) --- Astronauts Brent W. Jett Jr. (left) and Koichi Wakata work with the Protein Crystal Growth (PCG) experiment at the Single Locker Thermal Enclosure System (STES) on the Space Shuttle Endeavour’s mid-deck. Jett, making his first flight in space, served as the crew’s pilot, while Wakata served as a mission specialist. Wakata, also a first time Shuttle crew member, represents Japan’s National Space Development Agency (NASDA).
61C-05-036 (12-18 Jan. 1986) --- U.S. Representative Bill Nelson (Democrat - Florida), STS-61C payload specialist, prepares to photograph individual samples in the Handheld Protein Crystal Growth Experiment (HPCG) on Columbia's middeck. The operations involve the use of four pieces of equipment to attempt the growth of 60 different types of crystals -- 12 by means of dialysis and 48 via the vapor diffusion method. The photo was used by members of the STS-61C crew at their Jan. 23, 1986, Post-Flight Press Conference.
As the most abundant protein in the circulatory system albumin contributes 80% to colloid osmotic blood pressure. Albumin is also chiefly responsible for the maintenance of blood pH. It is located in every tissue and bodily secretion, with extracellular protein comprising 60% of total albumin. Perhaps the most outstanding property of albumin is its ability to bind reversibly to an incredible variety of ligands. It is widely accepted in the pharmaceutical industry that the overall distribution, metabolism, and efficiency of many drugs are rendered ineffective because of their unusually high affinity for this abundant protein. An understanding of the chemistry of the various classes of pharmaceutical interactions with albumin can suggest new approaches to drug therapy and design. Principal Investigator: Dan Carter/New Century Pharmaceuticals
Type II restriction enzymes, such as Eco R1 endonulease, present a unique advantage for the study of sequence-specific recognition because they leave a record of where they have been in the form of the cleaved ends of the DNA sites where they were bound. The differential behavior of a sequence -specific protein at sites of differing base sequence is the essence of the sequence-specificity; the core question is how do these proteins discriminate between different DNA sequences especially when the two sequences are very similar. Principal Investigator: Dan Carter/New Century Pharmaceuticals
iss058e001945 (Jan. 3, 2019) --- Expedition 58 Flight Engineer and astronaut Anne McClain of NASA peers into a microscope and takes photographs for the Protein Crystal Growth-16 experiment that is exploring therapies for Parkinson's disease.
iss058e001880 (Jan. 2, 2019) --- NASA astronaut and Expedition 58 Flight Engineer Anne McClain works inside the Unity module conducting research operations for the Protein Crystal Experiment-16 that is exploring therapies for Parkinson's disease.
iss057e106232 (Nov. 26, 2018) --- Commander Alexander Gerst uses a uses a pipette to transfer a protein solution into the Protein Crystal Growth Card for an experiment observing protein crystals associated with Parkinson’s disease to potentially improve treatments on Earth.
Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein crystallography. It is used as an excellent model system for better understanding parameters involved in microgravity experiments with data from laboratory experiments to study the equilibrium rate of hanging drop experiments in microgravity.
Dan Carter and Charles Sisk center a Lysozyme Protein crystal grown aboard the USML-2 shuttle mission. Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein crystallography. It is used as an excellent model system for better understanding parameters involved in microgravity crystal growth experiments. The goal is to compare kinetic data from microgravity experiments with data from laboratory experiments to study the equilibrium.
iss064e039017 (March 2, 2021) --- NASA astronaut Michael Hopkins loads protein crystallography plates with protein solutions for the Phase II Real-time Protein Crystal Growth experiment, a space commercialization study, that could benefit the pharmaceutical and biotechnology industries.
iss064e038995 (March 2, 2021) --- NASA astronaut and Expedition 64 Flight Engineer Michael Hopkins loads protein crystallography plates with protein solutions for the Phase II Real-time Protein Crystal Growth experiment, a space commercialization study, that could benefit the pharmaceutical and biotechnology industries.
iss064e039273 (March 2, 2021) --- NASA astronaut and Expedition 64 Flight Engineer Michael Hopkins loads protein crystallography plates with protein solutions for the Phase II Real-time Protein Crystal Growth experiment, a space commercialization study, that could benefit the pharmaceutical and biotechnology industries.
iss047e055611 (4/11/2016) --- A view of the JAXA Protein Crystal Growth (PCG) Demo Sample, in the Japanese Experiment Module (JEM) Pressurized Module (JPM) aboard the International space Station (ISS). The objective of JAXA High Quality Protein Crystal Growth Demonstration Experiment (JAXA PCG-Demo) is to grow high quality protein crystals in microgravity.
iss047e055613 (4/11/2016) --- A view of the JAXA Protein Crystal Growth (PCG) Demo Sample, in the Japanese Experiment Module (JEM) Pressurized Module (JPM) aboard the International space Station (ISS). The objective of JAXA High Quality Protein Crystal Growth Demonstration Experiment (JAXA PCG-Demo) is to grow high quality protein crystals in microgravity.
iss057e106231 (Nov. 26, 2018) --- European Space Agency (ESA) asrtonaut Alexander Gerst uses a uses a pipette to transfer a protein solution into the Protein Crystal Growth Card for an experiment observing protein crystals associated with Parkinson’s disease to potentially improve treatments on Earth. Crystallization of LRRK2 Under Microgravity Conditions-2 (CASIS PCG 16) evaluates growth of Leucine-rich repeat kinase 2 (LRRK2) protein crystals in microgravity. LRRK2 is implicated in Parkinson’s disease, but crystals of the protein grown on Earth are too small and compact to study. Detailed analysis of larger, space-grown crystals can define the protein’s exact shape and morphology and help scientists better understand the disease’s pathology.
iss065e085491 (June 3, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Megan McArthur loads protein crystallography plates with protein solutions for the Real-time Protein Crystal Growth experiment. The biotechnology study demonstrates new methods for producing high-quality protein crystals in microgravity. Results may help identify new targets and develop better drugs to treat a variety of diseases on Earth and advance the commercialization of low-Earth orbit.
iss065e144296 (June 14, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Megan McArthur loads protein crystallography plates with protein solutions, mixes them with custom salt solutions, then seals and transfers the plates for incubation for the Real-Time Protein Crystal Growth-2 experiment. The biotechnology study looks at new ways to produce high-quality protein crystals which could lead to new disease therapies on Earth.
iss057e106419 (Nov. 30, 2018) --- Samples from the Protein Crystal Growth Card are examined using a microscope for an experiment observing protein crystals associated with Parkinson’s disease to potentially improve treatments on Earth. Crystallization of LRRK2 Under Microgravity Conditions-2 (CASIS PCG 16) evaluates growth of Leucine-rich repeat kinase 2 (LRRK2) protein crystals in microgravity. LRRK2 is implicated in Parkinson’s disease, but crystals of the protein grown on Earth are too small and compact to study. Detailed analysis of larger, space-grown crystals can define the protein’s exact shape and morphology and help scientists better understand the disease’s pathology.
iss057e106417 (Nov. 30, 2018) --- Samples from the Protein Crystal Growth Card are examined using a microscope for an experiment observing protein crystals associated with Parkinson’s disease to potentially improve treatments on Earth. Crystallization of LRRK2 Under Microgravity Conditions-2 (CASIS PCG 16) evaluates growth of Leucine-rich repeat kinase 2 (LRRK2) protein crystals in microgravity. LRRK2 is implicated in Parkinson’s disease, but crystals of the protein grown on Earth are too small and compact to study. Detailed analysis of larger, space-grown crystals can define the protein’s exact shape and morphology and help scientists better understand the disease’s pathology.
This photo shows an individual cell from the Handheld Diffusion Test Cell (HH-DTC) apparatus flown on the Space Shuttle. Similar cells will be used in the Observable Protein Crystal Growth Apparatus (OPCGA) to be operated aboard the International Space Station (ISS). The principal investigator is Dr. Alex McPherson of the University of California, Irvine. Each individual cell comprises two sample chambers with a rotating center section that isolates the two from each other until the start of the experiment and after it is completed. The cells are made from optical-quality quartz glass to allow photography and interferometric observations. Each cell has a small light-emitting diode and lens to back-light the solution. In protein crystal growth experiments, a precipitating agent such as a salt solution is used to absorb and hold water but repel the protein molecules. This increases the concentration of protein until the molecules nucleate to form crystals. This cell is one of 96 that make up the experiment module portion of the OPCGA.
This photo shows the Handheld Diffusion Test Cell (HH-DTC) apparatus flown on the Space Shuttle. Similar cells (inside the plastic box) will be used in the Observable Protein Crystal Growth Apparatus (OPCGA) to be operated aboard the International Space Station (ISS). The principal investigator is Dr. Alex McPherson of the University of California, Irvine. Each individual cell comprises two sample chambers with a rotating center section that isolates the two from each other until the start of the experiment and after it is completed. The cells are made from optical-quality quartz glass to allow photography and interferometric observations. Each cell has a small light-emitting diode and lens to back-light the solution. In protein crystal growth experiments, a precipitating agent such as a salt solution is used to absorb and hold water but repel the protein molecules. This increases the concentration of protein until the molecules nucleate to form crystals. This cell is one of 96 that make up the experiment module portion of the OPCGA.
iss065e154962 (July 6, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Megan McArthur sets up a microscope to view protein crystal samples for the Real-time Protein Crystal Growth-2 experiment. The biotechnology study looks at new ways to produce high-quality protein crystals which could lead to new disease therapies on Earth.
Eddie Snell (standing), Post-Doctoral Fellow the National Research Council (NRC),and Marc Pusey of Marshall Space Flight Center (MSFC) use a reciprocal space mapping diffractometer for marcromolecular crystal quality studies. The diffractometer is used in mapping the structure of marcromolecules such as proteins to determine their structure and thus understand how they function with other proteins in the body. This is one of several analytical tools used on proteins crystalized on Earth and in space experiments. Photo credit: NASA/Marshall Space Flight Center (MSFC)
Christiane Gumera, right, a student at Stanton College Preparatory High School in Jacksonville, AL, examines a protein sample while preparing an experiment for flight on the International Space Station (ISS). Merle Myers, left, a University of California, Irvine, researcher, prepares to quick-freeze protein samples in nitrogen. The proteins are in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be anlyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)
iss057e074528 (Nov. 9, 2018) --- NASA astronaut Serena Auñón-Chancellor is pictured in the Japanese Kibo lab module mixing protein crystal samples to help scientists understand how they work. BioServe Protein Crystalography-1 (BPC-1) seeks to demonstrate the feasibility of conducting protein crystal growth in real time aboard the International Space Station. Crew members add solutions to the hardware, observe the crystals that form and adjust for follow-on experiments.
iss047e105727 (5/10/2016) --- Photographic documentation of the Japan Aerospace Exploration Agency (JAXA) High Quality Protein Crystal Growth (PCG) Removal. The PCG-Canister Bags were removed from the Cell Biology Experiment Facility (CBEF) and the Protein Crystallization Research Facility (PCRF) before being stowed for return on SpX-8. The JAXA PCG-Demo investigation crystallizes proteins using the counter-diffusion technique and permeation method that minimizes impurities, forming high-quality crystals for use in medical studies and ecological applications.
The human body contains more than 100,000 types of proteins, each providing information related to our health. Studying these proteins by crystallizing them helps researchers learn more about the body and potential disease treatments. Additionally, researchers have discovered that growing crystals in microgravity allows for slower growth and higher quality crystals. Hopkins and Glover both worked on the RTPCG-2 protein crystal experiment to advance new drug discoveries.
Eddie Snell, Post-Doctoral Fellow the National Research Council (NRC) uses a reciprocal space mapping diffractometer for macromolecular crystal quality studies. The diffractometer is used in mapping the structure of macromolecules such as proteins to determine their structure and thus understand how they function with other proteins in the body. This is one of several analytical tools used on proteins crystallized on Earth and in space experiments. Photo credit: NASA/Marshall Space Flight Center (MSFC)
Mission Specialist George (Pinky) D. Nelson uses a 35 mm camera to photograph a protein crystal grown during the STS-26 Protein Crystal Growth (PCG-II-01) experiment. The protein crystal growth (PCG) carrier is shown deployed from the PCG Refrigerator/Incubator Mocule (R/IM) located in the middeck forward locker. The R/IM contained three Vapor Diffusion Apparatus (VDS) trays (one of which is shown). A total of sixty protein crystal samples were processed during the STS-26 mission.
STS066-13-029 (3-14 Nov 1994) --- On the Space Shuttle Atlantis' mid-deck, astronaut Scott E. Parazynski, mission specialist, works at one of two areas onboard the Shuttle which support the Protein Crystal Growth (PCG) experiment. This particular section is called the Vapor Diffusion Apparatus (VDA), housed in a Single Locker Thermal Enclosure (STES). Together with the Crystal Observation System, housed in the Thermal Enclosure System (COS/TES) the VDA represents the continuing research into the structures of proteins and other macromolecules such as viruses. In addition to using the microgravity of space to grow high-quality protein crystals for structural analyses, the experiments are expected to help develop technologies and methods to improve the protein crystallization process on Earth as well as in space.
iss056e142862_alt (8/13/2018) --- Astronaut Ricky Arnold prepares samples for the Barrios Protein Crystal Growth (Barrios PCG) experiment in the Maintenance Work Area (MWA) of the International Space Station (ISS). The Barrios PCG experiment defined an approach for optimizing protein crystallization conditions on orbit, eliminating the need to return samples to the ground during the optimization phase, which has the potential for saving substantial time and money on future PCG investigations in microgravity.
iss051e052364 (6/2/2017) --- European Space Agency (ESA) astronaut Thomas Pesquet works to remove Canisters from the Protein Crystallization Research Facility (PCRF) for handover to cosmonaut Oleg Novitskiy. The image was taken in the Kibo Japanese Experiment Pressurized Module (JPM) during Japan Aerospace Exploration Agency (JAXA) Protein Crystal Growth (PCG) and Kristallizator experiment operations (OPS).
iss064e011289 (12/8/2020) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
iss068e075597 (3/17/2023) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
iss056e142865_alt (8/13/2018) --- Astronaut Ricky Arnold prepares samples for the Barrios Protein Crystal Growth (Barrios PCG) experiment in the Maintenance Work Area (MWA) of the International Space Station (ISS). The Barrios PCG experiment defined an approach for optimizing protein crystallization conditions on orbit, eliminating the need to return samples to the ground during the optimization phase, which has the potential for saving substantial time and money on future PCG investigations in microgravity.
iss056e142871_alt (8/13/2018) --- Astronaut Ricky Arnold prepares samples for the Barrios Protein Crystal Growth (Barrios PCG) experiment in the Maintenance Work Area (MWA) of the International Space Station (ISS). The Barrios PCG experiment defined an approach for optimizing protein crystallization conditions on orbit, eliminating the need to return samples to the ground during the optimization phase, which has the potential for saving substantial time and money on future PCG investigations in microgravity.
iss064e011283 (12/8/2020) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
iss064e011287 (12/8/2020) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
iss068e075601 (3/17/2023) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
iss051e052377 96/2/2017) --- European Space Agency (ESA) astronaut Thomas Pesquet and cosmonaut Fyodor Yurchikhin pose with Canister Bags during handover of Canisters removed from the Protein Crystallization Research Facility (PCRF. The image was taken in the Kibo Japanese Experiment Pressurized Module (JPM) during Japan Aerospace Exploration Agency (JAXA) Protein Crystal Growth (PCG) and Kristallizator experiment operations (OPS).
iss068e075598 (3/17/2023) --- A view of the Ice Cubes Experiment Cube #6 – Kirara mission, in the Columbus module aboard the International Space Station (ISS). The Ice Cubes Experiment Cube #6 – Kirara is an in-orbit validation of an incubator for growing protein crystals in microgravity for future commercial business opportunities. This first demonstration mission includes proteins for seven different companies and research institutes.
The Commercial Vapor Diffusion Apparatus will be used to perform 128 individual crystal growth investigations for commercial and science research. These experiments will grow crystals of several different proteins, including HIV-1 Protease Inhibitor, Glycogen Phosphorylase A, and NAD Synthetase. The Commercial Vapor Diffusion Apparatus supports multiple commercial investigations within a controlled environment. The goal of the Commercial Protein Crystal Growth payload on STS-95 is to grow large, high-quality crystals of several different proteins of interest to industry, and to continue to refine the technology and procedures used in microgravity for this important commercial research.
Space Shuttle Discovery (STS-26) astronauts George Nelson, John Lounge, and Richard Covey are pictured training on protein crystal growth (PCG) experiment in Marshall's Building 4708's clean room.
iss060e053892 -- Expedition 60 flight engineer Christina Koch of NASA uses a microscope to observe and photograph growing protein crystal samples as part of the Microgravity Crystals experiment aboard the International Space Station.
Front view of Observable Protein Crystal Growth Apparatus (OPCGA) experiment residing in a Thermal Enclosure System (TES). Principal Investigator is Alexander McPherson. First flight plarned for ISS.
Joel Kearns viewing a laboratory demonstration of the Observable Protein Crystal Growth Apparatus (OPCGA) experiment module. Principal Investigator is Alexander McPherson. First flight plarned for ISS.
Space shuttle STS-121 FIT (Fly Immunity and Tumors) payload. Using Drosophila (fruit fly) to complete the experiments. Computer screen showing green fluorescent protein used to visualize blood cells in Drosophila (fruit fly).
iss008e18236 (3/3/2004) --- Close-up view of a PromISS 3 (Protein Crystal Growth Monitoring by Digital Holographic Microscope) experiment sample. The experiment was conducted by the Expedition 8 crew in the Microgravity Science Glovebox (MSG) located in the Destiny U.S. Laboratory aboard the International Space Station (ISS).
ISS008-E-05015 (27 October 2003) --- European Space Agency (ESA) astronaut Pedro Duque of Spain works with the Cervantes mission experiment PROMISS in the Microgravity Science Glovebox (MSG) in the Destiny laboratory on the International Space Station (ISS). This experiment will investigate the growth processes of proteins in weightless conditions.
ISS008-E-05029 (27 October 2003) --- European Space Agency (ESA) astronaut Pedro Duque of Spain works with the Cervantes mission experiment PROMISS in the Microgravity Science Glovebox (MSG) in the Destiny laboratory on the International Space Station (ISS). This experiment will investigate the growth processes of proteins in weightless conditions.
KENNEDY SPACE CENTER, FLA. - The Microgravity Science Laboratory-1 (MSL-1) Spacelab module is installed into the payload bay of the Space Shuttle Orbiter Columbia in Orbiter Processing Facility 1. The Spacelab long crew transfer tunnel that leads from the orbiter's crew airlock to the module is also aboard, as well as the Hitchhiker Cryogenic Flexible Diode (CRYOFD) experiment payload, which is attached to the right side of Columbia's payload bay. During the scheduled 16-day STS-83 mission, the MSL-1 will be used to test some of the hardware, facilities and procedures that are planned for use on the International Space Station while the flight crew conducts combustion, protein crystal growth and materials processing experiments.
KENNEDY SPACE CENTER, FLA. - The Microgravity Science Laboratory-1 (MSL-1) Spacelab module is installed into the payload bay of the Space Shuttle Orbiter Columbia in Orbiter Processing Facility 1. The Spacelab long crew transfer tunnel that leads from the orbiter's crew airlock to the module is also aboard, as well as the Hitchhiker Cryogenic Flexible Diode (CRYOFD) experiment payload, which is attached to the right side of Columbia's payload bay. During the scheduled 16-day STS-83 mission, the MSL-1 will be used to test some of the hardware, facilities and procedures that are planned for use on the International Space Station while the flight crew conducts combustion, protein crystal growth and materials processing experiments.
These Vapor Diffusion Apparatus (VDA) trays were first flown in the Thermal Enclosure System (TES) during the USMP-2 (STS-62) mission. Each tray can hold 20 protein crystal growth chambers. Each chamber contains a double-barrel syringe; one barrel holds protein crystal solution and the other holds precipitant agent solution. During the microgravity mission, a torque device is used to simultaneously retract the plugs in all 20 syringes. The two solutions in each chamber are then mixed. After mixing, droplets of the combined solutions are moved onto the syringe tips so vapor diffusion can begin. During the length of the mission, protein crystals are grown in the droplets. Shortly before the Shuttle's return to Earth, the experiment is deactivated by retracting the droplets containing protein crystals, back into the syringes.
iss050e058807 (3/17/2017) --- A view of European Space Agency (ESA) astronaut Thomas Pesquet, during Protein Crystal Growth (PCG) -5 hardware deactivation and stow, from Microgravity Experiment Research Locker Incubator (MERLIN) on Expedite the Processing of Experiments to the Space Station (EXPRESS) Rack 5. The Microgravity Growth of Crystalline Monoclonal Antibodies for Pharmaceutical Applications (CASIS-PCG-5) investigation crystallizes a monoclonal antibody developed by Merck Research Labs. Microgravity enables the growth of extremely high-quality crystals, which allow scientists to study the proteins’ structure, improve drug delivery, manufacturing, and developing better methods for storing these biological molecules.
iss050e058812 (3/17/2017) --- A view of European Space Agency (ESA) astronaut Thomas Pesquet, during Protein Crystal Growth (PCG) -5 hardware deactivation and stow, from Microgravity Experiment Research Locker Incubator (MERLIN) on Expedite the Processing of Experiments to the Space Station (EXPRESS) Rack 5. The Microgravity Growth of Crystalline Monoclonal Antibodies for Pharmaceutical Applications (CASIS-PCG-5) investigation crystallizes a monoclonal antibody developed by Merck Research Labs. Microgravity enables the growth of extremely high-quality crystals, which allow scientists to study the proteins’ structure, improve drug delivery, manufacturing, and developing better methods for storing these biological molecules.
iss050e058802 (3/17/2017) --- A view of European Space Agency (ESA) astronaut Thomas Pesquet, during Protein Crystal Growth (PCG) -5 hardware deactivation and stow, from Microgravity Experiment Research Locker Incubator (MERLIN) on Expedite the Processing of Experiments to the Space Station (EXPRESS) Rack 5. The Microgravity Growth of Crystalline Monoclonal Antibodies for Pharmaceutical Applications (CASIS-PCG-5) investigation crystallizes a monoclonal antibody developed by Merck Research Labs. Microgravity enables the growth of extremely high-quality crystals, which allow scientists to study the proteins’ structure, improve drug delivery, manufacturing, and developing better methods for storing these biological molecules.
iss053e037294 (9/22/2017) --- A view of the Advanced Nano Step Cartridge Installation into the Solution Crystallization Observation Facility (SCOF) aboard the International Space Station (ISS). The Effects of Impurities on Perfection of Protein Crystals, Partition Functions, and Growth Mechanisms (Advanced Nano Step) experiment monitors and records how the incorporation of specific impurity molecules affect the development and quality of protein crystals, as they grow in a quartz cell aboard the ISS.
A Memphis student working at the University of Alabama in Huntsville prepares samples for the first protein crystal growth experiments plarned to be performed aboard the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)
On the Space Shuttle Atlantis' mid-deck, astronaut Joseph R. Tanner, mission specialist, works at area amidst several lockers onboard the Shuttle which support the Protein Crystal Growth (PCG) experiment. This particular section is called the Crystal Observation System, housed in the Thermal Enclosure System (COS/TES). Together with the Vapor Diffusion Apparatus (VDA), housed in a Single Locker Thermal Enclosure (SLTES) which is out of frame, the Cos/TES represents the continuing research into the structures of proteins and other macromolecules such as viruses.
Horse Serum Albumin crystals grown during the USML-1 (STS-50) mission's Protein Crystal Growth Glovebox Experiment. These crystals were grown using a vapor diffusion technique at 22 degrees C. The crystals were allowed to grow for nine days while in orbit. Crystals of 1.0 mm in length were produced. The most abundant blood serum protein, regulates blood pressure and transports ions, metabolites, and therapeutic drugs. Principal Investigator was Edward Meehan.
On the Space Shuttle Orbiter Atlantis' middeck, Astronaut Joseph R. Tarner, mission specialist, works at an area amidst several lockers which support the Protein Crystal Growth (PCG) experiment during the STS-66 mission. This particular section is called the Crystal Observation System, housed in the Thermal Enclosure System (COS/TES). Together with the Vapor Diffusion Apparatus (VDA), housed in Single Locker Thermal Enclosure (SLTES), the COS/TES represents the continuing research into the structure of proteins and other macromolecules such as viruses.
iss053e039947 (9/22/2017) --- NASA astronaut Mark T. Vande Hei is shown with the Advanced Nano Step Cartridge in the Solution Crystallization Observation Facility (SCOF) during installation. The Effects of Impurities on Perfection of Protein Crystals, Partition Functions, and Growth Mechanisms (Advanced Nano Step) experiment monitors and records how the incorporation of specific impurity molecules affect the development and quality of protein crystals, as they grow in a quartz cell aboard the International Space Station (ISS).
A Memphis student working at the University of Alabama in Huntsville prepares samples for the first protein crystal growth experiments plarned to be performed aboard the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)
iss065e066486 (May 24, 2021) --- Expedition 65 Flight Engineer Thomas Pesquet of ESA (European Space Agency) services samples for the Real Time Protein Crystal Growth experiment. The biotechnology study demonstrates new methods for producing high-quality protein crystals in microgravity to potentially develop better drugs to treat a variety of diseases on Earth and advance the commercialization of space.
Memphis students working at the University of Alabama in Huntsville prepare samples for the first protein crystal growth experiments plarned to be performed aboard the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)
iss065e066472 (May 24, 2021) --- Expedition 65 Flight Engineer Thomas Pesquet of ESA (European Space Agency) services samples for the Real Time Protein Crystal Growth experiment. The biotechnology study demonstrates new methods for producing high-quality protein crystals in microgravity to potentially develop better drugs to treat a variety of diseases on Earth and advance the commercialization of space.
iss065e006581 (April 26, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Michael Hopkins packs components of the Real-time Protein Crystal Growth experiment for return and analysis on Earth. The biotechnology study seeks to demonstrate new methods for producing high-quality protein crystals in microgravity and identify possible targets for drugs to treat diseases on Earth.
iss065e084430 (May 31, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Shane Kimbrough sets up a microscope in the U.S. Destiny laboratory module to observe and photograph samples for the Real-Time Protein Crystal Growth experiment. Results have implications for biotechnology and pharmaceutical companies on Earth and may advance the commercialization of space.
STS085-324-007 (7 - 19 August 1997) --- Astronaut Kent V. Rominger, pilot, uses a tool to deactivate the Protein Crystal Growth (PCG) experiment on the mid-deck of the Space Shuttle Discovery near the end of the 12-day STS-85 flight.
iss062e087805 (March 11, 2020) --- NASA astronaut and Expedition 62 Flight Engineer Jessica Meir collects Protein Crystal Growth-10 experiment hardware for stowage inside JAXA's (Japan Aerospace Exploration Agency) Kibo laboratory module.
Cindy Barnes of University Space Research Association (USRA) at NASA's Marshall Space Flight Center pipettes a protein solution in preparation to grow crystals as part of NASA's structural biology program. Research on Earth helps scientists define conditions and specimens they will use in space experiments.
Onboard Space Shuttle Columbia (STS-73) Mission Specialists Catherine Cady Coleman works at the glovebox facility in support of the Protein Crystal Growth Glovebox (PCG-GBX) experiment in the United States Microgravity Laboratory 2 (USML-2) Spacelab science module.
iss065e081518 (May 31, 2021) --- NASA astronaut and Expedition 65 Flight Engineer Shane Kimbrough sets up a microscope in the U.S. Destiny laboratory module to observe and photograph samples for the Real-Time Protein Crystal Growth experiment. Results have implications for biotechnology and pharmaceutical companies on Earth and may advance the commercialization of space.
iss055e024521 (April 18, 2018) --- Flight Engineer Drew Feustel holds a bag containing samples that had been collected, documented and inspected for the Protein Crystal Growth-9 experiment. Feustel was in the Cupola as the International Space Station was orbiting over southern Mexico near the Guatemalan border.
iss056e142859 (Aug. 13, 2018) --- Expedition 56 Commander Drew Feustel is inside the Harmony module working on the Protein Crystal Growth-13 experiment which is seeking to fine-tune the research process in space and help public and private organizations deliver results and benefits sooner.
iss056e142857 (Aug. 13, 2018) --- Expedition 56 Commander Drew Feustel is inside the Harmony module working on the Protein Crystal Growth-13 experiment which is seeking to fine-tune the research process in space and help public and private organizations deliver results and benefits sooner.
iss060e035164 (Aug. 12, 2019) --- Expedition 60 Flight Engineer Christina Koch of NASA conducts research operations for a protein crystal growth experiment. The research is investigating the production of antibody therapies with a longer shelf-life to benefit humans on Earth and in space.
iss067e378812 (Sept. 21, 2022) --- Expedition 67 Flight Engineer and ESA (European Space Agency) astronaut Samantha Cristoforetti works inside the Microgravity Science Glovebox removing hardware that supported the Ring Sheared Drop experiment. The fluid physics study observes the formation of destructive protein clusters that may be responsible for neurodegenerative diseases such as Alzheimer’s.
iss056e142855 (Aug. 13, 2018) --- Expedition 56 Commander Drew Feustel is inside the Harmony module working on the Protein Crystal Growth-13 experiment which is seeking to fine-tune the research process in space and help public and private organizations deliver results and benefits sooner.
Astronaut Wendy B. Lawrence, flight engineer and mission specialist for STS-67, scribbles notes on the margin of a checklist while monitoring an experiment on the Space Shuttle Endeavour's mid-deck. The experiment is the Protein Crystal Growth (PCG), which takes up locker space near the Commercial Materials Dispersion Apparatus Instruments Technology Associates Experiment (CMIX).
High school students screen crystals of various proteins that are part of the ground-based work that supports Alexander McPherson's protein crystal growth experiment. The students also prepared and stored samples in the Enhanced Gaseous Nitrogen Dewar, which was launched on the STS-98 mission for delivery to the ISS. The crystals grown on the ground will be compared with crystals grown in orbit. Participants include Joseph Negron, of Terry Parker High School, Jacksonville, Florida; Megan Miskowski (shown), of Ridgeview High School, Orange Park, Florida; and Sam Swank, of Fletcher High School, Neptune Beach, Florida. The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center.
High school students screen crystals of various proteins that are part of the ground-based work that supports Alexander McPherson's protein crystal growth experiment. The students also prepared and stored samples in the Enhanced Gaseous Nitrogen Dewar, which was launched on the STS-98 mission for delivery to the ISS. The crystals grown on the ground will be compared with crystals grown in orbit. Participants include Joseph Negron, of Terry Parker High School, Jacksonville, Florida; Megan Miskowski, of Ridgeview High School, Orange Park, Florida; and Sam Swank (shown), of Fletcher High School, Neptune Beach, Florida. The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center.
High school students screen crystals of various proteins that are part of the ground-based work that supports Alexander McPherson's protein crystal growth experiment. The students also prepared and stored samples in the Enhanced Gaseous Nitrogen Dewar, which was launched on the STS-98 mission for delivery to the ISS. The crystals grown on the ground will be compared with crystals grown in orbit. Participants include Joseph Negron (shown), of Terry Parker High School, Jacksonville, Florida; Megan Miskowski, of Ridgeview High School, Orange Park, Florida; and Sam Swank, of Fletcher High School, Neptune Beach, Florida. The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center.
Experiments to seek solutions for a range of biomedical issues are at the heart of several investigations that will be hosted by the Commercial Instrumentation Technology Associates (ITA), Inc. Biomedical Experiments (CIBX-2) payload. CIBX-2 is unique, encompassing more than 20 separate experiments including cancer research, commercial experiments, and student hands-on experiments from 10 schools as part of ITA's ongoing University Among the Stars program. Valerie Cassanto of ITA checks the Canadian Protein Crystallization Experiment (CAPE) carried by STS-86 to Mir in 1997. The experiments are sponsored by NASA's Space Product Development Program (SPD).
Kim Nelson, left, of Sandalwood High School in Jacksonville, FL, helps Steven Nepowada, right, of Terry Parker High School in Jacksonville, practice loading a protein sample into a thermos-like container, known as Dewar. Students from Jacksonville worked with researchers from NASA/Marshall Space Flight Center (MSFC), as well as universities, in Huntsville, AL, on an experiment for the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)
In August 2001, principal investigator Jeanne Becker sent human ovarian tumor cells to the International Space Station (ISS) aboard the STS-105 mission. The tumor cells were cultured in microgravity for a 14 day growth period and were analyzed for changes in the rate of cell growth and synthesis of associated proteins. In addition, they were evaluated for the expression of several proteins that are the products of oncogenes, which cause the transformation of normal cells into cancer cells. This photo, which was taken by astronaut Frank Culbertson who conducted the experiment for Dr. Becker, shows two cell culture bags containing LN1 ovarian carcinoma cell cultures.
In the Destiny laboratory aboard the International Space Station (ISS), European Space Agency (ESA) astronaut Pedro Duque of Spain is seen working at the Microgravity Science Glovebox (MSG). He is working with the PROMISS experiment, which will investigate the growth processes of proteins during weightless conditions. The PROMISS is one of the Cervantes program of tests (consisting of 20 commercial experiments). The MSG is managed by NASA's Marshall Space Flight Center (MSFC).
ISS040-E-130230 (9 Sept. 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, installs a microscope for the Cell Mechanosensing-2 experiment in the Kibo laboratory of the International Space Station. The Japanese experiment, which is conducted in Kibo’s Kobairo rack, seeks to identify gravity sensors in cells that may change the expression of key proteins and genes and allowing muscles to atrophy in microgravity.
ISS008-E-05026 (27 October 2003) --- European Space Agency (ESA) astronaut Pedro Duque (left) of Spain works with the Cervantes mission experiment PROMISS in the Microgravity Science Glovebox (MSG) in the Destiny laboratory on the International Space Station (ISS). This experiment will investigate the growth processes of proteins in weightless conditions. Astronaut Edward T. Lu, Expedition 7 NASA ISS science officer and flight engineer, is visible at right.
European Space Agency astronaut Alexander Gerst,Expedition 40 flight engineer,installs a microscope for the Cell Mechanosensing-2 experiment in the Kibo laboratory of the International Space Station. The Japanese experiment,which is conducted in Kibos Kobairo rack,seeks to identify gravity sensors in cells that may change the expression of key proteins and genes and allowing muscles to atrophy in microgravity.
ISS040-E-130232 (9 Sept. 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, installs a microscope for the Cell Mechanosensing-2 experiment in the Kibo laboratory of the International Space Station. The Japanese experiment, which is conducted in Kibo’s Kobairo rack, seeks to identify gravity sensors in cells that may change the expression of key proteins and genes and allowing muscles to atrophy in microgravity.
Astronaut Franklin R. Chang-Diaz, payload commander, ponders the elements of a model representing the Commercial Protein Crystal Growth (CPCG) experiment. This flight of the experiment marks the first joint United States--Latin America effort in this discipline. The project brings together a small team of investigators from Costa Rica (Chang-Diaz's native land), Chile, and the United States.
ISS040-E-130233 (9 Sept. 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, installs a microscope for the Cell Mechanosensing-2 experiment in the Kibo laboratory of the International Space Station. The Japanese experiment, which is conducted in Kibo’s Kobairo rack, seeks to identify gravity sensors in cells that may change the expression of key proteins and genes and allowing muscles to atrophy in microgravity.
ISS043E087335 (04/03/2015) --- ESA (European Space Agency) astronaut Samantha Cristoforetti works to retrieve samples for the Aniso Tubule experiment from the Cell Biology Experiment Facility (CBEF) on Apr. 3, 2015. Aniso Tubule examines growth modifications of Arabidopsis hypocotyls in space. Scientists will analyze the changes in dynamics of cortical microtubules and microtubule associated proteins with a fluorescence microscope.
iss061e068057 (Dec. 9, 2019) --- NASA astronaut and Expedition 61 Flight Engineer Jessica Meir installs the Bone Densitometer in an EXPRESS (EXpedite the PRocessing of Experiments to Space Station) rack located inside the Japanese Kibo laboratory module. The Bone Densitometer enables the imaging of rodent bones for the Rodent Research-19 experiment that is investigating two proteins that may prevent muscle and bone loss in space.
ISS040-E-130231 (9 Sept. 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, installs a microscope for the Cell Mechanosensing-2 experiment in the Kibo laboratory of the International Space Station. The Japanese experiment, which is conducted in Kibo’s Kobairo rack, seeks to identify gravity sensors in cells that may change the expression of key proteins and genes and allowing muscles to atrophy in microgravity.
ISS007-E-17880 (20 October 2003) --- European Space Agency (ESA) astronaut Pedro Duque of Spain prepares to set up the Cervantes program of tests (consisting of 20 commercial experiments) by starting with the Microgravity Science Glovebox (MSB) in the Destiny laboratory on the International Space Station (ISS). Duque is working on the PROMISS experiment, which will investigate the growth processes of proteins during weightless conditions.