jsc2022e083572 (10/20/20220 --- A preflight image of a beating Engineered Heart Tissue (EHT) for the Engineered Heart Tissues-2 investigation. The tissue is fabricated between two posts, one flexible and one rigid. In the flexible post, you can see a square magnet. This magnet enables researchers to measure tissue function using an underlying magnetic sensor, giving real time tissue function data. Image courtesy of Johns Hopkins University.
jsc2022e083014 (10/26/2022) --- A preflight image of a beating Engineered Heart Tissue (EHT) for A Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues-2) investigation. The tissue is fabricated between two posts, one flexible and one rigid. In the flexible post, a square magnet is seen. This magnet enables researchers to measure tissue function using an underlying magnetic sensor, giving real time tissue function data. Image courtesy of Johns Hopkins University.
jsc2022e083015 (10/26/2022) --- A preflight image of tissue chambers loaded into the plate habitat (pHAB) for A Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues-2) investigation. Each tissue chamber contains six tissues and is placed over magnetic sensors on a circuit board to measure contractile function of the Engineered Heart Tissues (EHTs). Image courtesy of Johns Hopkins University.
jsc2022e072970 (9/22/2022) --- A preflight view of 3D heart cells generated by microscale tissue engineering. ISS: Engineering Stem Cell-Derived Cardiac Microtissues with Metabolic Regulators in Space to Promote Cardiomyocyte Maturation (Project EAGLE) grows 3D cultures of heart cells on the International Space Station. What is learned could help scientists establish a functional heart tissue model that mimics heart disease and can be used to test new drugs. Image courtesy of Parvin Forghani, Ph.D., Emory University.
iss064e015250 (Dec. 24, 2020) --- NASA astronaut and Expedition 64 Flight Engineer Kate Rubins works inside the Life Sciences Glovebox (LSG) servicing engineered heart tissue samples for the Cardinal Heart study that seeks to understand space-caused cell and tissue abnormalities. The LSG is located inside Japan's Kibo laboratory module.
An image of the cardiac tissue chip with an array of cardiac tissues as part of MVP Cell-09. This investigation aims to understand the cellular and molecular mechanisms by which Streptococcus pneumoniae, the leading cause of community-acquired pneumonia damages heart tissue. Bacteria are generally more active and virulent in the unique conditions of space. Investigators hypothesize microgravity may amplify the effects of Streptococcus pneumoniae on heart cells, exaggerating important cell responses that would not be detected on Earth. Credit. University of Alabama at Birmingham.
iss062e115367 (3/26/2020) --- Tissue chambers shown during media exchanges and tissue fixations of the Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissue) investigation inside the Life Sciences Glovebox (LSG) in the Japanese Experiment Module (JEM) aboard the International Space Station (ISS). The Engineered Heart Tissues research model could be an effective tool for better understanding cardiac function in response to external factors which would be useful for drug development and other applications related to cardiac dysfunction on Earth.
iss062e115350 (3/26/2020) --- Tissue chambers shown during media exchanges and tissue fixations of the Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissue) investigation inside the Life Sciences Glovebox (LSG) in the Japanese Experiment Module (JEM) aboard the International Space Station (ISS). The Engineered Heart Tissues research model could be an effective tool for better understanding cardiac function in response to external factors which would be useful for drug development and other applications related to cardiac dysfunction on Earth.
iss062e115333 (3/26/2020) --- Tissue chambers shown during media exchanges and tissue fixations of the Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissue) investigation inside the Life Sciences Glovebox (LSG) in the Japanese Experiment Module (JEM) aboard the International Space Station (ISS). The Engineered Heart Tissues research model could be an effective tool for better understanding cardiac function in response to external factors which would be useful for drug development and other applications related to cardiac dysfunction on Earth.
An image of a cardiac tissue chip positioned inside the MVP’s cell culture chamber as part of MVP Cell-09. This investigation aims to understand the cellular and molecular mechanisms by which Streptococcus pneumoniae, the leading cause of community-acquired pneumonia damages heart tissue. Bacteria are generally more active and virulent in the unique conditions of space. Investigators hypothesize microgravity may amplify the effects of Streptococcus pneumoniae on heart cells, exaggerating important cell responses that would not be detected on Earth. Credit. University of Alabama at Birmingham.
iss048e045908 (7/29/2016) --- NASA astronaut Kate Rubins is photographed at the Microgravity Science Glovebox (MSG) as she works to change the media in the Multiwell BioCells for the Heart Cells experiment. Effects of Microgravity on Stem Cell-Derived Cardiomyocytes (Heart Cells) studies the human heart, specifically how heart muscle tissue, contracts, grows and changes (gene expression) in microgravity and how those changes vary between subjects
jsc2022e072971 (9/22/2022) --- A preflight view 3D heart cells in suspension culture. ISS: Engineering Stem Cell-Derived Cardiac Microtissues with Metabolic Regulators in Space to Promote Cardiomyocyte Maturation (Project EAGLE) grows 3D cultures of heart cells on the International Space Station. What is learned could help scientists establish a functional heart tissue model that mimics heart disease and can be used to test new drugs. Image courtesy of Parvin Forghani, Ph.D., Emory University.
iss048e045065 (7/27/2016) --- NASA astronaut Kate Rubins pauses for a photo while using the Microscope to conduct Heart Cells experiment operations. Effects of Microgravity on Stem Cell-Derived Cardiomyocytes (Heart Cells) studies the human heart, specifically how heart muscle tissue, contracts, grows and changes (gene expression) in microgravity and how those changes vary between subjects.
jsc2022e083016 (10/26/2022) --- A preflight image of tissue chambers loaded into the plate habitat (pHAB) for A Human iPSC-based 3D Microphysiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues-2) investigation. The tissue chambers are placed inside the pHAB lid, creating a fully enclosed system for functional measurements and long-term tissue culture in microgravity. Image courtesy of Johns Hopkins University.
iss064e020036 (Jan. 5, 2021) --- Expedition 64 Flight Engineer and NASA astronaut Kate Rubins explores the space-caused aging and weakening of heart muscles that astronauts experience for the Cardinal Heart study. The experiment uses engineered heart tissue samples and is taking place inside the Life Sciences Glovebox located in the Japanese Kibo laboratory module.
iss064e020062 (1/5/2021) --- A close-up view of the cell culture media change in a chamber containing engineered heart tissues as part of the Cardinal Heart experiment aboard the International Space Station (ISS). This investigation seeks to help scientists understand the aging and weakening of heart muscles to provide new treatments for humans on Earth and astronauts in space.
iss074e0518242 (April 23, 2026) --- NASA astronaut and Expedition 74 flight engineer Jessica Meir processes samples of heart stem cells and bacteria that cause pneumonia using a portable glovebag inside the International Space Station's Harmony module. Research in microgravity may give doctors a clearer understanding of how infectious disease processes damage heart tissue at the cellular and molecular level. These insights could lead to advanced treatments for heart conditions both on Earth and in space. Credit: NASA/Chris Williams
jsc2024e038395 (6/5/2024) --- Live human heart tissue bioprinted with Redwire's BioFabrication Facility aboard the International Space Station. The tissue was successfully returned to Earth in April 2024. Results of the Redwire Cardiac Bioprinting Investigation (BFF-Cardiac) could advance technologies for producing organs and tissues in lieu of donated organs for transplant. The investigation also improves 3D printing, with the goal of giving the crew the ability to print material like foods and medicines on demand for future long-duration space missions. Image courtesy of Redwire.
A SpaceX Falcon 9 rocket, with the company’s Dragon spacecraft atop, is secured in the vertical position at NASA Kennedy Space Center’s Launch Complex 39A on March 13, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A
A SpaceX Falcon 9 rocket, with the company’s Dragon spacecraft atop, is raised to a vertical position at NASA Kennedy Space Center’s Launch Complex 39A on March 13, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A.
A SpaceX Falcon 9 rocket, with the company’s Dragon spacecraft atop, is secured in the vertical position at NASA Kennedy Space Center’s Launch Complex 39A on March 13, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A.
Seen here is a up-close view of the SpaceX Dragon spacecraft atop the company’s Falcon 9 rocket in the vertical position at NASA’s Kennedy Space Center in Florida on March 14, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A.
Seen here is a up-close view of the SpaceX Dragon spacecraft atop the company’s Falcon 9 rocket in the vertical position at NASA’s Kennedy Space Center in Florida on March 14, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A.
Lisa Freed and Gordana Vunjak-Novakovic, both of the Massachusetts Institute of Technology (MIT), have taken the first steps toward engineering heart muscle tissue that could one day be used to patch damaged human hearts. Cells isolated from very young animals are attached to a three-dimensional polymer scaffold, then placed in a NASA bioreactor. The cells do not divide, but after about a week start to cornect to form a functional piece of tissue. Here, a transmission electron micrograph of engineered tissue shows a number of important landmarks present in functional heart tissue: (A) well-organized myofilaments (Mfl), z-lines (Z), and abundant glycogen granules (Gly); and (D) intercalcated disc (ID) and desmosomes (DES). The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). Credit: MIT
Lisa Freed and Gordana Vunjak-Novakovic, both of the Massachusetts Institute of Technology (MIT), have taken the first steps toward engineering heart muscle tissue that could one day be used to patch damaged human hearts. Cells isolated from very young animals are attached to a three-dimensional polymer scaffold, then placed in a NASA bioreactor. The cells do not divide, but after about a week start to cornect to form a functional piece of tissue. Functionally connected heart cells that are capable of transmitting electrical signals are the goal for Freed and Vunjak-Novakovic. Electrophysiological recordings of engineered tissue show spontaneous contractions at a rate of 70 beats per minute (a), and paced contractions at rates of 80, 150, and 200 beats per minute respectively (b, c, and d). The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). Credit: NASA and MIT.
An image shows the culture medium bags during preflight testing as part of the MVP Cell-09. This investigation aims to understand the cellular and molecular mechanisms by which Streptococcus pneumoniae, the leading cause of community-acquired pneumonia damages heart tissue. Bacteria are generally more active and virulent in the unique conditions of space. Investigators hypothesize microgravity may amplify the effects of Streptococcus pneumoniae on heart cells, exaggerating important cell responses that would not be detected on Earth. Credit. University of Alabama at Birmingham.
iss062e120658 (April 2, 2020) --- NASA astronaut and Expedition 62 Flight Engineer conducts cardiac research in the Life Sciences Glovebox located in the Japanese Kibo laboratory module. The Engineered Heart Tissues investigation could promote a better understanding of cardiac function in microgravity which would be useful for drug development and other applications related to heart conditions on Earth.
iss062e115369 (March 26, 2020) --- NASA astronaut and Expedition 62 Flight Engineer Jessica Meir conducts cardiac research in the Life Sciences Glovebox located in the Japanese Kibo laboratory module. The Engineered Heart Tissues investigation could promote a better understanding of cardiac function in microgravity which would be useful for drug development and other applications related to heart conditions on Earth.
iss068e076142 (March 23, 2021) --- UAE (United Arab Emirates) astronaut and Expedition 68 Flight Engineer Sultan Alneyadi works in the Kibo laboratory module's Life Science Glovebox. Alneyadi was conducting research for the Cardinal Heart 2.0 investigation that is testing clinically-approved pharmaceutical drugs to reverse the negative effects on heart cells and tissues caused by prolonged exposure to the space environment.
iss074e0491074 (April 16, 2026) --- NASA astronaut and Expedition 74 flight engineer Chris Williams configures research hardware inside a portable glovebag for a biotechnology investigation exploring how bacteria affect heart tissue in the microgravity environment. Results from the MVP (Multi-use Variable-g Platform) Cell-09 experiment could lead to advanced methods for preventing or treating heart damage in humans living on and off the Earth. Credit: NASA/Jessica Meir
iss074e0490731 (April 16, 2026) --- NASA astronaut and Expedition 74 flight engineer Jack Hathaway configures research hardware inside a portable glovebag for a biotechnology investigation exploring how bacteria affect heart tissue in the microgravity environment. Results from the MVP (Multi-use Variable-g Platform) Cell-09 experiment could lead to advanced methods for preventing or treating heart damage in humans living on and off the Earth. Credit: ESA/Sophie Adenot
iss074e0490661 (April 16, 2026) --- NASA astronaut and Expedition 74 flight engineer Jessica Meir configures research hardware inside a portable glovebag for a biotechnology investigation exploring how bacteria affect heart tissue in the microgravity environment. Results from the MVP (Multi-use Variable-g Platform) Cell-09 experiment could lead to advanced methods for preventing or treating heart damage in humans living on and off the Earth. Credit: ESA/Sophie Adenot
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
Creating a golden streak in the night sky, a SpaceX Falcon 9 rocket carrying the Dragon spacecraft soars upward after liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
Seen here is an up-close view of the SpaceX Dragon spacecraft atop the company’s Falcon 9 rocket after being raised to a vertical position at NASA’s Kennedy Space Center in Florida on March 13, 2023, in preparation for the 27th commercial resupply services launch to the International Space Station. The mission will deliver new science investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. Liftoff is scheduled for 8:30 p.m. EDT on Tuesday, March 14, from Kennedy’s Launch Complex 39A.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The heart of the bioreactor is the rotating wall vessel, shown without its support equipment. Volume is about 125 mL. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket’s first stage separates from the Dragon spacecraft a few minutes after liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
Creating a golden streak in the night sky, a SpaceX Falcon 9 rocket soars upward after liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
The SpaceX Falcon 9 rocket carrying the Dragon spacecraft lifts off from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th commercial resupply services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. Dragon will deliver more than 6,000 pounds of cargo, including a variety of NASA investigations, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
A SpaceX Falcon 9 rocket soars upward after its liftoff from Launch Complex 39A at NASA’s Kennedy Space Center in Florida on March 14, 2023, on the company’s 27th Commercial Resupply Services mission for the agency to the International Space Station. Liftoff was at 8:30 p.m. EDT. The Dragon spacecraft will deliver more than 6,000 pounds of science and research, supplies, and equipment to the crew aboard the space station, including the final two experiments comprising the National Institutes for Health and International Space Station National Laboratory’s Tissue Chips in Space initiative, Cardinal Heart 2.0 and Engineered Heart Tissues-2. The spacecraft is expected to spend about a month attached to the orbiting outpost before it returns to Earth with research and return cargo, splashing down off the coast of Florida.
Paul Ducheyne, a principal investigator in the microgravity materials science program and head of the University of Pernsylvania's Center for Bioactive Materials and Tissue Engineering, is leading the trio as they use simulated microgravity to determine the optimal characteristics of tiny glass particles for growing bone tissue. The result could make possible a much broader range of synthetic bone-grafting applications. Even in normal gravity, bioactive glass particles enhance bone growth in laboratory tests with flat tissue cultures. Ducheyne and his team believe that using the bioactive microcarriers in a rotating bioreactor in microgravity will produce improved, three-dimensional tissue cultures. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: NASA and University of Pennsylvania Center for Bioactive Materials and Tissue Engineering.
The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues currently being cultured in rotating bioreactors by investigators
The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues currently being cultured in rotating bioreactors by investigators.
iss062e115343 (March 26, 2020) --- NASA astronaut and Expedition 62 Flight Engineer Jessica Meir conducts cardiac research inside the Life Sciences Glovebox, a biology research facility located in Japan's Kibo laboratory module. The Engineered Heart Tissues investigation is exploring cardiac function in weightlessness that may provide new drug developments for astronauts and Earthlings.
Organ chips are roughly the size of a USB drive and could be used to predict how an individual might respond to a variety of stressors, such as radiation or medical treatments, including pharmaceuticals. Made with human cells, the chips mimic how tissues, such as the brain, heart, liver, or dozens of other organs, work. NASA research will focus on validating and leveraging these models to assess the impacts of deep space stressors on astronauts’ health.
iss064e020142 (Jan. 5, 2021) --- Expedition 64 Flight Engineer and NASA astronaut Kate Rubins loads engineered heart tissue samples into a science freezer for preservation and later analysis. The science freezer, located in the Japanese Kibo laboratory module, is known as the Minus Eighty-Degree Laboratory Freezer for ISS (MELFI) and maintains experiment samples at ultra-cold temperatures throughout a mission.
iss062e115355 (March 26, 2020) --- NASA astronaut and Expedition 62 Flight Engineer Jessica Meir conducts cardiac research inside the Life Sciences Glovebox, a biology research facility located in Japan's Kibo laboratory module. The Engineered Heart Tissues investigation is exploring cardiac function in weightlessness that may provide new drug developments for astronauts and Earthlings.
Dr. Lisa E. Freed of the Massachusetts Institute of Technology and her colleagues have reported that initially disc-like specimens tend to become spherical in space, demonstrating that tissues can grow and differentiate into distinct structures in microgravity. The Mir Increment 3 (Sept. 16, 1996 - Jan. 22, 1997) samples were smaller, more spherical, and mechanically weaker than Earth-grown control samples. These results demonstrate the feasibility of microgravity tissue engineering and may have implications for long human space voyages and for treating musculoskeletal disorders on earth. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Cells cultured on Earth (left) typically settle quickly on the bottom of culture vessels due to gravity. In microgravity (right), cells remain suspended and aggregate to form three-dimensional tissue. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Astronaut John Blaha replaces an exhausted media bag and filled waste bag with fresh bags to continue a bioreactor experiment aboard space station Mir in 1996. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. This image is from a video downlink. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC).
Electronics control module for the NASA Bioreactor. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
This prostate cancer construct was grown during NASA-sponsored bioreactor studies on Earth. Cells are attached to a biodegradable plastic lattice that gives them a head start in growth. Prostate tumor cells are to be grown in a NASA-sponsored Bioreactor experiment aboard the STS-107 Research-1 mission in 2002. Dr. Leland Chung of the University of Virginia is the principal investigator. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: NASA and the University of Virginia.
Close-up view of the interior of a NASA Bioreactor shows the plastic plumbing and valves (cylinders at right center) to control fluid flow. The rotating wall vessel is at top center. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Diagram depicts the importance of cell-cell communication as central to the understanding of cancer growth and progression, the focus of the NASA bioreactor demonstration system (BDS-05) investigation. Microgravity studies will allow us to unravel the signaling and communication between these cells with the host and potential development of therapies for the treatment of cancer metastasis. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: Emory University.
Leland W. K. Chung (left), Director, Molecular Urology Therapeutics Program at the Winship Cancer Institute at Emory University, is principal investigator for the NASA bioreactor demonstration system (BDS-05). With him is Dr. Jun Shu, an assistant professor of Orthopedics Surgery from Kuming Medical University China. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: Emory University.
Exterior view of the NASA Bioreactor Engineering Development Unit flown on Mir. The rotating wall vessel is behind the window on the face of the large module. Control electronics are in the module at left; gas supply and cooling fans are in the module at back. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Interior of a Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell and with thermal blankets partially removed. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Laptop computer sits atop the Experiment Control Computer for a NASA Bioreactor. The flight crew can change operating conditions in the Bioreactor by using the graphical interface on the laptop. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Close-up view of the interior of a NASA Bioreactor shows the plastic plumbing and valves (cylinders at center) to control fluid flow. A fresh nutrient bag is installed at top; a flattened waste bag behind it will fill as the nutrients are consumed during the course of operation. The drive chain and gears for the rotating wall vessel are visible at bottom center center. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Interior view of the gas supply for the NASA Bioreactor. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Cell constructs grown in a rotating bioreactor on Earth (left) eventually become too large to stay suspended in the nutrient media. In the microgravity of orbit, the cells stay suspended. Rotation then is needed for gentle stirring to replenish the media around the cells.
Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
The schematic depicts the major elements and flow patterns inside the NASA Bioreactor system. Waste and fresh medium are contained in plastic bags placed side-by-side so the waste bag fills as the fresh medium bag is depleted. The compliance vessel contains a bladder to accommodate pressure transients that might damage the system. A peristolic pump moves fluid by squeezing the plastic tubing, thus avoiding potential contamination. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Biotechnology Specimen Temperature Controller (BSTC) will cultivate cells until their turn in the bioreactor; it can also be used in culturing experiments that do not require the bioreactor. The BSTC comprises four incubation/refrigeration chambers individually set at 4 to 50 deg. C (near-freezing to above body temperature). Each chamber holds three rugged tissue chamber modules (12 total), clear Teflon bags holding 30 ml of growth media, all positioned by a metal frame. Every 7 to 21 days (depending on growth rates), an astronaut uses a shrouded syringe and the bags' needleless injection ports to transfer a few cells to a fresh media bag, and to introduce a fixative so that the cells may be studied after flight. The design also lets the crew sample the media to measure glucose, gas, and pH levels, and to inspect cells with a microscope. The controller is monitored by the flight crew through a 23-cm (9-inch) color computer display on the face of the BSTC. This view shows the BTSC with the front panel open. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
For 5 days on the STS-70 mission, a bioreactor cultivated human colon cancer cells, which grew to 30 times the volume of control specimens grown on Earth. This significant result was reproduced on STS-85 which grew mature structures that more closely match what are found in tumors in humans. Shown here, clusters of cells slowly spin inside a bioreactor. On Earth, the cells continually fall through the buffer medium and never hit bottom. In space, they are naturally suspended. Rotation ensures gentle stirring so waste is removed and fresh nutrient and oxygen are supplied. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Biotechnology Specimen Temperature Controller (BSTC) will cultivate cells until their turn in the bioreactor; it can also be used in culturing experiments that do not require the bioreactor. The BSTC comprises four incubation/refrigeration chambers individually set at 4 to 50 degreesC (near-freezing to above body temperature). Each chamber holds three rugged tissue chamber modules (12 total), clear Teflon bags holding 30 ml of growth media, all positioned by a metal frame. Every 7 to 21 days (depending on growth rates), an astronaut uses a shrouded syringe and the bags' needleless injection ports to transfer a few cells to a fresh media bag, and to introduce a fixative so that the cells may be studied after flight. The design also lets the crew sample the media to measure glucose, gas, and pH levels, and to inspect cells with a microscope. The controller is monitored by the flight crew through a 23-cm (9-inch) color computer display on the face of the BSTC. This view shows the BTSC with the front panel open. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.
Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101825 for a version with major elements labeled, and No. 0103180 for an operational schematic. 0101816
Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101816 for a version without labels, and No. 0103180 for an operational schematic.
Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101824 for a version with labels, and No. 0103180 for an operational schematic.
Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101823 for a version without labels, and No. 0103180 for an operational schematic.
A SpaceX Falcon 9 rocket soars into the sky after lifting off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020. The rocket is carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket soars into the sky after lifting off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020. The rocket is carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket soars into the sky after lifting off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020. The rocket is carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket lifts off from Launch Complex 39A at Kennedy Space Center in Florida at 11:17 a.m. EST on Dec. 6, 2020, carrying the uncrewed cargo Dragon spacecraft on its journey to the International Space Station for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission. Dragon will deliver more than 6,400 pounds of science investigations and cargo to the orbiting laboratory. The mission marks the first launch for SpaceX under NASA’s CRS-2 contract.
A SpaceX Falcon 9 rocket and cargo Dragon spacecraft stand poised for launch moments before liftoff at Kennedy Space Center’s Launch Complex 39A in Florida on Dec. 6, 2020, for NASA and SpaceX’s 21st Commercial Resupply Services (CRS-21) mission to the International Space Station. The first launch for SpaceX under NASA’s CRS-2 contract, the mission blasted off the pad at 11:17 a.m. EST.