Diagram depicts the importance of cell-cell communication as central to the understanding of cancer growth and progression, the focus of the NASA bioreactor demonstration system (BDS-05) investigation. Microgravity studies will allow us to unravel the signaling and communication between these cells with the host and potential development of therapies for the treatment of cancer metastasis. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: Emory University.

ISS018-E-034090 (20 Feb. 2009) --- Astronaut Sandra Magnus, Expedition 18 flight engineer, uses a communication system near the Cell Biology Experiment Facility (CBEF) in the Kibo laboratory of the International Space Station.

Cells cultured on Earth (left) typically settle quickly on the bottom of culture vessels due to gravity. In microgravity (right), cells remain suspended and aggregate to form three-dimensional tissue. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Cell constructs grown in a rotating bioreactor on Earth (left) eventually become too large to stay suspended in the nutrient media. In the microgravity of orbit, the cells stay suspended. Rotation then is needed for gentle stirring to replenish the media around the cells.

This prostate cancer construct was grown during NASA-sponsored bioreactor studies on Earth. Cells are attached to a biodegradable plastic lattice that gives them a head start in growth. Prostate tumor cells are to be grown in a NASA-sponsored Bioreactor experiment aboard the STS-107 Research-1 mission in 2002. Dr. Leland Chung of the University of Virginia is the principal investigator. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: NASA and the University of Virginia.

Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Interior of a Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Interior view of the gas supply for the NASA Bioreactor. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Laptop computer sits atop the Experiment Control Computer for a NASA Bioreactor. The flight crew can change operating conditions in the Bioreactor by using the graphical interface on the laptop. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Close-up view of the interior of a NASA Bioreactor shows the plastic plumbing and valves (cylinders at right center) to control fluid flow. The rotating wall vessel is at top center. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Electronics control module for the NASA Bioreactor. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Leland W. K. Chung (left), Director, Molecular Urology Therapeutics Program at the Winship Cancer Institute at Emory University, is principal investigator for the NASA bioreactor demonstration system (BDS-05). With him is Dr. Jun Shu, an assistant professor of Orthopedics Surgery from Kuming Medical University China. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: Emory University.

Close-up view of the interior of a NASA Bioreactor shows the plastic plumbing and valves (cylinders at center) to control fluid flow. A fresh nutrient bag is installed at top; a flattened waste bag behind it will fill as the nutrients are consumed during the course of operation. The drive chain and gears for the rotating wall vessel are visible at bottom center center. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Exterior view of the NASA Bioreactor Engineering Development Unit flown on Mir. The rotating wall vessel is behind the window on the face of the large module. Control electronics are in the module at left; gas supply and cooling fans are in the module at back. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Biotechnology Refrigerator that preserves samples for use in (or after culturing in) the NASA Bioreactor. The unit is shown extracted from a middeck locker shell and with thermal blankets partially removed. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Lisa Freed and Gordana Vunjak-Novakovic, both of the Massachusetts Institute of Technology (MIT), have taken the first steps toward engineering heart muscle tissue that could one day be used to patch damaged human hearts. Cells isolated from very young animals are attached to a three-dimensional polymer scaffold, then placed in a NASA bioreactor. The cells do not divide, but after about a week start to cornect to form a functional piece of tissue. Functionally connected heart cells that are capable of transmitting electrical signals are the goal for Freed and Vunjak-Novakovic. Electrophysiological recordings of engineered tissue show spontaneous contractions at a rate of 70 beats per minute (a), and paced contractions at rates of 80, 150, and 200 beats per minute respectively (b, c, and d). The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). Credit: NASA and MIT.

For 5 days on the STS-70 mission, a bioreactor cultivated human colon cancer cells, which grew to 30 times the volume of control specimens grown on Earth. This significant result was reproduced on STS-85 which grew mature structures that more closely match what are found in tumors in humans. Shown here, clusters of cells slowly spin inside a bioreactor. On Earth, the cells continually fall through the buffer medium and never hit bottom. In space, they are naturally suspended. Rotation ensures gentle stirring so waste is removed and fresh nutrient and oxygen are supplied. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

iss062e098366 (3/17/2020) --- A View of the ISSET-Nanoracks-Mission Discovery 4 investigation aboard the International Space Station (ISS). The Flatworm regeneration sub-investigation studies how cells in flatworms communicate with each other to enable them to regenerate in microgravity. This experiment may allow investigators to learn why certain species are able to regenerate.

The Pathfinder research aircraft's solar cell arrays are prominently displayed as it touches down on the bed of Rogers Dry Lake at the Dryden Flight Research Center, Edwards, California, following a test flight. The solar arrays covered more than 75 percent of Pathfinder's upper wing surface, and provided electricity to power its six electric motors, flight controls, communications links and a host of scientific sensors.

iss062e098361 (3/17/2020) --- A View of the ISSET-Nanoracks-Mission Discovery 4 investigation aboard the International Space Station (ISS). The Flatworm regeneration sub-investigation studies how cells in flatworms communicate with each other to enable them to regenerate in microgravity. This experiment may allow investigators to learn why certain species are able to regenerate.

Antonia Jaramillo, NASA Communications, moderates a What’s On Board Science Briefing on June 2, 2021, at Kennedy Space Center in Florida for SpaceX’s 22nd Commercial Resupply Services mission for NASA to the International Space Station. On screen, principal investigator Dr. Thomas Boothby describes the Cell Science-04 experiment that will be delivered to the space station. Cell Science-04 will research the effects of microgravity on tardigrades, more commonly known as water bears. The results could advance understanding of the stress factors affecting humans in space. The SpaceX Falcon 9 rocket with the Dragon capsule atop is scheduled to launch at 1:29 p.m. EDT on Thursday, June 3, from the center’s Launch Complex 39A. Dragon will deliver more than 7,300 pounds of cargo and science experiments to the space station.

Astronaut John Blaha replaces an exhausted media bag and filled waste bag with fresh bags to continue a bioreactor experiment aboard space station Mir in 1996. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. This image is from a video downlink. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC).

The heart of the bioreactor is the rotating wall vessel, shown without its support equipment. Volume is about 125 mL. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues currently being cultured in rotating bioreactors by investigators

In this photo, science tubes containing bacterial cells are being prepped for cleaning as part of the Dynamics of Microbiomes in Space (DynaMoS) experiment inside the Space Station Processing Facility at NASA’s Kennedy Space Center in Florida on July 11, 2022. Once clean, the cells will be moved to a larger space for continued growth in order to generate enough biomass to inoculate the soil that will be used in the experiment. The DynaMoS experiment will launch on SpaceX’s 25th cargo resupply services mission to examine how microgravity affects metabolic interactions in communities of soil microbes. This will help NASA understand the function of soil microorganisms in space versus on Earth and how they can be used to enhance plant growth for crew consumption during long-duration missions to the Moon and Mars. SpaceX’s Falcon 9 rocket and Dragon spacecraft are scheduled to lift off from Kennedy’s Launch Complex 39A at 8:44 p.m. EDT on Thursday, July 14.

The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues currently being cultured in rotating bioreactors by investigators.

Biotechnology Specimen Temperature Controller (BSTC) will cultivate cells until their turn in the bioreactor; it can also be used in culturing experiments that do not require the bioreactor. The BSTC comprises four incubation/refrigeration chambers individually set at 4 to 50 deg. C (near-freezing to above body temperature). Each chamber holds three rugged tissue chamber modules (12 total), clear Teflon bags holding 30 ml of growth media, all positioned by a metal frame. Every 7 to 21 days (depending on growth rates), an astronaut uses a shrouded syringe and the bags' needleless injection ports to transfer a few cells to a fresh media bag, and to introduce a fixative so that the cells may be studied after flight. The design also lets the crew sample the media to measure glucose, gas, and pH levels, and to inspect cells with a microscope. The controller is monitored by the flight crew through a 23-cm (9-inch) color computer display on the face of the BSTC. This view shows the BTSC with the front panel open. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Lisa Freed and Gordana Vunjak-Novakovic, both of the Massachusetts Institute of Technology (MIT), have taken the first steps toward engineering heart muscle tissue that could one day be used to patch damaged human hearts. Cells isolated from very young animals are attached to a three-dimensional polymer scaffold, then placed in a NASA bioreactor. The cells do not divide, but after about a week start to cornect to form a functional piece of tissue. Here, a transmission electron micrograph of engineered tissue shows a number of important landmarks present in functional heart tissue: (A) well-organized myofilaments (Mfl), z-lines (Z), and abundant glycogen granules (Gly); and (D) intercalcated disc (ID) and desmosomes (DES). The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). Credit: MIT

Biotechnology Specimen Temperature Controller (BSTC) will cultivate cells until their turn in the bioreactor; it can also be used in culturing experiments that do not require the bioreactor. The BSTC comprises four incubation/refrigeration chambers individually set at 4 to 50 degreesC (near-freezing to above body temperature). Each chamber holds three rugged tissue chamber modules (12 total), clear Teflon bags holding 30 ml of growth media, all positioned by a metal frame. Every 7 to 21 days (depending on growth rates), an astronaut uses a shrouded syringe and the bags' needleless injection ports to transfer a few cells to a fresh media bag, and to introduce a fixative so that the cells may be studied after flight. The design also lets the crew sample the media to measure glucose, gas, and pH levels, and to inspect cells with a microscope. The controller is monitored by the flight crew through a 23-cm (9-inch) color computer display on the face of the BSTC. This view shows the BTSC with the front panel open. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Operators in the Engine Research Building’s Central Control Room at the National Advisory Committee for Aeronautics (NACA) Lewis Flight Propulsion Laboratory. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful collection of compressors and exhausters located in the central portion of the basement provided process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities. This photograph was taken just after a major upgrade to the control room in 1948. The panels on the wall contain rudimentary floor plans of the different Engine Research Building sections with indicator lights and instrumentation for each test cell. The process air equipment included 12 exhausters, four compressors, a refrigeration system, cooling water, and an exhaust system. The operators in the control room kept in contact with engineers running the process air system and those conducting the tests in the test cells. The operators also coordinated with the local power companies to make sure enough electricity was available to operate the powerful compressors and exhausters.

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, the Optical Payload for Lasercomm Science, or OPALS, experiment has been unpacked in a test cell at a Space Station Processing Facility offline laboratory. The optical technology demonstration experiment arrived from the agency’s Jet Propulsion Laboratory in Pasadena, Calif. NASA will use the International Space Station to test OPALS’ communications technology that could dramatically improve spacecraft communications, enhance commercial missions and strengthen transmission of scientific data. The experiment is slated to fly later this year aboard a SpaceX Dragon commercial resupply mission to the space station. The mission is expected to run 90 days after installation on the outside of the station. For more information about OPALS, visit: http://go.nasa.gov/10MMPDO. Photo credit: NASA/Jim Grossmann

S70-34902 (14 April 1970) --- Several persons important to the Apollo 13 mission, at consoles in the Mission Operations Control Room (MOCR) of the Mission Control Center (MCC). Seated at consoles, from left to right, are astronauts Donald K. Slayton, director of flight crew operations; astronaut Jack R. Lousma, Shift 3 spacecraft communicator; and astronaut John W. Young, commander of the Apollo 13 backup crew. Standing, left to right, are astronaut Tom K. Mattingly II, who was replaced as Apollo 13 command module pilot after it was learned he may come down with measles, and astronaut Vance D. Brand, Shift 2 spacecraft communicator. Several hours earlier, in the late evening hours of April 13, crew members of the Apollo 13 mission reported to MCC that trouble had developed with an oxygen cell on their spacecraft.

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, technicians uncover and check the Optical Payload for Lasercomm Science, or OPALS, experiment in a test cell at a Space Station Processing Facility offline laboratory. The optical technology demonstration experiment arrived from the agency’s Jet Propulsion Laboratory in Pasadena, Calif. NASA will use the International Space Station to test OPALS’ communications technology that could dramatically improve spacecraft communications, enhance commercial missions and strengthen transmission of scientific data. The experiment is slated to fly later this year aboard a SpaceX Dragon commercial resupply mission to the space station. The mission is expected to run 90 days after installation on the outside of the station. For more information about OPALS, visit: http://go.nasa.gov/10MMPDO. Photo credit: NASA/Jim Grossmann

CAPE CANAVERAL, Fla. --At NASA’s Kennedy Space Center in Florida, technicians uncover and check the Optical Payload for Lasercomm Science, or OPALS, experiment in a test cell at a Space Station Processing Facility offline laboratory. The optical technology demonstration experiment arrived from the agency’s Jet Propulsion Laboratory in Pasadena, Calif. NASA will use the International Space Station to test OPALS’ communications technology that could dramatically improve spacecraft communications, enhance commercial missions and strengthen transmission of scientific data. The experiment is slated to fly later this year aboard a SpaceX Dragon commercial resupply mission to the space station. The mission is expected to run 90 days after installation on the outside of the station. For more information about OPALS, visit: http://go.nasa.gov/10MMPDO. Photo credit: NASA/Jim Grossmann

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, the Optical Payload for Lasercomm Science, or OPALS, experiment has been uncovered in a test cell at a Space Station Processing Facility offline laboratory. The optical technology demonstration experiment arrived from the agency’s Jet Propulsion Laboratory in Pasadena, Calif. NASA will use the International Space Station to test OPALS’ communications technology that could dramatically improve spacecraft communications, enhance commercial missions and strengthen transmission of scientific data. The experiment is slated to fly later this year aboard a SpaceX Dragon commercial resupply mission to the space station. The mission is expected to run 90 days after installation on the outside of the station. For more information about OPALS, visit: http://go.nasa.gov/10MMPDO. Photo credit: NASA/Jim Grossmann

S70-34902 (14 April 1970) --- Several persons important to the Apollo 13 mission, at consoles in the Mission Operations Control Room (MOCR) of the Mission Control Center (MCC). Seated at consoles, from left to right, are astronauts Donald K. Slayton, director of flight crew operations; astronaut Jack R. Lousma, Shift 3 spacecraft communicator; and astronaut John W. Young, commander of the Apollo 13 backup crew. Standing, left to right, are astronaut Tom K. Mattingly II, who was replaced as Apollo 13 command module pilot after it was learned he may come down with measles, and astronaut Vance D. Brand, Shift 2 spacecraft communicator. Several hours earlier, in the late evening hours of April 13, crew members of the Apollo 13 mission reported to MCC that trouble had developed with an oxygen cell on their spacecraft.

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, technicians prepare to uncover and check the Optical Payload for Lasercomm Science, or OPALS, experiment in a test cell at a Space Station Processing Facility offline laboratory. The optical technology demonstration experiment arrived from the agency’s Jet Propulsion Laboratory in Pasadena, Calif. NASA will use the International Space Station to test OPALS’ communications technology that could dramatically improve spacecraft communications, enhance commercial missions and strengthen transmission of scientific data. The experiment is slated to fly later this year aboard a SpaceX Dragon commercial resupply mission to the space station. The mission is expected to run 90 days after installation on the outside of the station. For more information about OPALS, visit: http://go.nasa.gov/10MMPDO. Photo credit: NASA/Jim Grossmann

Ye Zhang, a project scientist at NASA’s Kennedy Space Center in Florida runs a test on a Gravite 3d clinostat device in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

A Gravite 3d clinostat is in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

S70-34904 (14 April 1970) --- Astronaut Alan B. Shepard Jr., prime crew commander of the Apollo 14 mission, monitors communications between the Apollo 13 spacecraft and Mission Control Center. He is seated at a console in the Mission Operations Control Room of the MCC, Manned Spacecraft Center. The main concern of the moment was action taken by the three Apollo 13 crewmen - astronauts James A. Lovell Jr., John L. Swigert Jr. and Fred W. Haise Jr. - to make corrections inside the spacecraft following discovery of an oxygen cell failure several hours earlier.

Ye Zhang, a project scientist at NASA’s Kennedy Space Center in Florida, makes adjustments to a Gravite 3d clinostat in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

A Gravite 3d clinostat undergoes a test in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

S70-34904 (14 April 1970) --- Astronaut Alan B. Shepard Jr., prime crew commander of the Apollo 14 mission, monitors communications between the Apollo 13 spacecraft and Mission Control Center. He is seated at a console in the Mission Operations Control Room of the MCC, Manned Spacecraft Center. The main concern of the moment was action taken by the three Apollo 13 crewmen - astronauts James A. Lovell Jr., John L. Swigert Jr. and Fred W. Haise Jr. - to make corrections inside the spacecraft following discovery of an oxygen cell failure several hours earlier.

Inside the Astrotech facility in Titusville, Florida, NASA's Tracking and Data Relay Satellite, TDRS-M, is undergoing final checkouts in a test cell behind a large door. The spacecraft soon will be encapsulated in its payload fairing, seen on the right. TDRS-M is the latest spacecraft destined for the agency's constellation of communications satellites that allows nearly continuous contact with orbiting spacecraft ranging from the International Space Station and Hubble Space Telescope to the array of scientific observatories. Liftoff atop a United Launch Alliance Atlas V rocket is scheduled to take place from Space Launch Complex 41 at Cape Canaveral Air Force Station at 9:02 a.m. EDT Aug. 3, 2017.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, programs the SciSpinner Microgravity Simulator in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Some experiments are being prepared for a test in the Airbus Random Positioning Machine in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility device was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Ye Zhang, a project scientist at NASA’s Kennedy Space Center in Florida, makes adjustments to a Gravite 3d clinostat in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Ye Zhang, a project scientist at NASA’s Kennedy Space Center in Florida, makes adjustments to a Gravite 3d clinostat in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

A Gravite 3d clinostat is in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Paul Ducheyne, a principal investigator in the microgravity materials science program and head of the University of Pernsylvania's Center for Bioactive Materials and Tissue Engineering, is leading the trio as they use simulated microgravity to determine the optimal characteristics of tiny glass particles for growing bone tissue. The result could make possible a much broader range of synthetic bone-grafting applications. Even in normal gravity, bioactive glass particles enhance bone growth in laboratory tests with flat tissue cultures. Ducheyne and his team believe that using the bioactive microcarriers in a rotating bioreactor in microgravity will produce improved, three-dimensional tissue cultures. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: NASA and University of Pennsylvania Center for Bioactive Materials and Tissue Engineering.

Operators in the Engine Research Building’s Central Control Room at the National Aeronautics and Space Administration (NASA) Lewis Research Center. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful a collection of compressors and exhausters located in the central portion of the basement provides process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities. The panels on the wall contain schematics with indicator lights and instrumentation for the atmospheric exhaust, altitude exhaust, refrigerated air, and process air systems. The process air equipment included twelve exhausters, four compressors, refrigeration system, cooling water, and an exhaust system. The operators in the control room kept in contact with engineers running the process air system and those conducting the tests in the test cells. The operators also coordinated with the local power companies to make sure enough electricity was available to operate the powerful compressors and exhausters.

Dr. Lisa E. Freed of the Massachusetts Institute of Technology and her colleagues have reported that initially disc-like specimens tend to become spherical in space, demonstrating that tissues can grow and differentiate into distinct structures in microgravity. The Mir Increment 3 (Sept. 16, 1996 - Jan. 22, 1997) samples were smaller, more spherical, and mechanically weaker than Earth-grown control samples. These results demonstrate the feasibility of microgravity tissue engineering and may have implications for long human space voyages and for treating musculoskeletal disorders on earth. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

The group of Japanese researchers of the Spacelab-J (SL-J) were thumbs-up in the Payload Operations Control Center (POCC) at the Marshall Space Flight Center after the successful launch of Space Shuttle Orbiter Endeavour that carried their experiments. The SL-J was a joint mission of NASA and the National Space Development Agency of Japan (NASDA) utilizing a marned Spacelab module. The mission conducted microgravity investigations in materials and life sciences. Materials science investigations covered such fields as biotechnology, electronic materials, fluid dynamics and transport phenomena, glasses and ceramics, metals and alloys, and acceleration measurements. Life sciences included experiments on human health, cell separation and biology, developmental biology, animal and human physiology and behavior, space radiation, and biological rhythms. Test subjects included the crew, Japanese koi fish (carp), cultured animal and plant cells, chicken embryos, fruit flies, fungi and plant seeds, frogs, and frog eggs. The POCC was the air/ground communications channel between the astronauts and ground control teams during the Spacelab missions. The Spacelab science operations were a cooperative effort between the science astronaut crew in orbit and their colleagues in the POCC. Spacelab-J was launched aboard the Space Shuttle Orbiter Endeavour on September 12, 1992.

The schematic depicts the major elements and flow patterns inside the NASA Bioreactor system. Waste and fresh medium are contained in plastic bags placed side-by-side so the waste bag fills as the fresh medium bag is depleted. The compliance vessel contains a bladder to accommodate pressure transients that might damage the system. A peristolic pump moves fluid by squeezing the plastic tubing, thus avoiding potential contamination. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Jeffrey Richards, a payload research and science coordinator on the LASSO contract at NASA’s Kennedy Space Center in Florida, prepares an experiment for a test in an Airbus Random Positioning Machine in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Jeffrey Richards, a payload research and science coordinator on the LASSO contract at NASA’s Kennedy Space Center in Florida, prepares an experiment for a test in an Airbus Random Positioning Machine in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Jeffrey Richards, a payload research and science coordinator on the LASSO contract at NASA’s Kennedy Space Center in Florida, prepares an experiment for a test in an Airbus Random Positioning Machine in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Ye Zhang, a project scientist at NASA’s Kennedy Space Center in Florida, makes adjustments to a Gravite 3d clinostat in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Researchers are in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. From left are Jonathan Gleeson, aerospace engineer on the LASSO contract; Jason Fischer, a research and development scientist on the LASSO contract; Ralph Nacca, aerospace flight systems; Jeffrey Richards, a payload research and science coordinator on the LASSO contract; and Dr. Ye Zhang, a project scientist. The microgravity simulation device was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Dr. Srujana Neelam, a NASA post-doctoral fellow observes samples on a confocal microscope in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020 with Jeffrey Richards, a payload research and science coordinator on the LASSO contract, Dr. Ye Zhang, a project scientist. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida, dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Srujana Neelam, a researcher working at NASA’s Kennedy Space Center in Florida dissects Arabidopsis thaliana plants from petri plates used in microgravity simulation devices in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building on Feb. 11, 2020. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Dr. Srujana Neelam, a NASA post-doctoral fellow observes samples on a confocal microscope in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020, with Jeffrey Richards, a payload research and science coordinator on the LASSO contract; and Dr. Ye Zhang, a project scientist. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

An engineer examines the Coherent Turbulence Rig in the Engine Research Building at the National Aeronautics and Space Administration (NASA) Lewis Research Center. Coherent turbulence occurs when waves of uniform size and alignment are present in airflow. Researchers at NASA Lewis were interested in determining the relation between the size of the waves and their heat transfer properties. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful a collection of compressors and exhausters located in the central portion of the basement provides process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities.

ISS022-E-011304 (15 Dec. 2009) --- NASA astronaut Jeffrey Williams, Expedition 22 commander, conducts a daily status check of the Advanced Plant Experiments on Orbit (APEX) experiment in the Kibo laboratory of the International Space Station. During each check, Williams looks for health and color of the plants, since the Cambium plants are removed from the Advanced Biological Research System (ABRS). When completed, the APEX-Cambium payload in conjunction with the NASA-sponsored Transgenic Arabidopsis Gene Expression System (TAGES) will determine the role of gravity in Cambium wood cell development and demonstrate non-destructive reporter gene technology and investigate spaceflight plant stress. APEX-Cambium provides NASA and the ISS community a permanent controlled environment capability to support growth of various organisms (i.e. whole plants).

Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101824 for a version with labels, and No. 0103180 for an operational schematic.

Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101825 for a version with major elements labeled, and No. 0103180 for an operational schematic. 0101816

Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101816 for a version without labels, and No. 0103180 for an operational schematic.

Bioreactor Demonstration System (BDS) comprises an electronics module, a gas supply module, and the incubator module housing the rotating wall vessel and its support systems. Nutrient media are pumped through an oxygenator and the culture vessel. The shell rotates at 0.5 rpm while the irner filter typically rotates at 11.5 rpm to produce a gentle flow that ensures removal of waste products as fresh media are infused. Periodically, some spent media are pumped into a waste bag and replaced by fresh media. When the waste bag is filled, an astronaut drains the waste bag and refills the supply bag through ports on the face of the incubator. Pinch valves and a perfusion pump ensure that no media are exposed to moving parts. An Experiment Control Computer controls the Bioreactor, records conditions, and alerts the crew when problems occur. The crew operates the system through a laptop computer displaying graphics designed for easy crew training and operation. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. See No. 0101823 for a version without labels, and No. 0103180 for an operational schematic.

Researchers are in the Microgravity Simulation Support Facility (MSSF) inside the Neil Armstrong Operations and Checkout building at NASA’s Kennedy Space Center in Florida on Feb. 11, 2020. In front, from left, are Jonathan Gleeson, aerospace engineer on the LASSO contract; Jason Fischer, a research and development scientist on the LASSO contract; and Ralph Nacca, aerospace flight systems. In back, from left, are Jeffrey Richards, a payload research and science coordinator on the LASSO contract; Dr. Ye Zhang, a project scientist; Dr. Srujana Neelam, a NASA post-doctoral fellow; Jessica Hellein, NASA intern; and Emily Keith, NASA intern. The facility was developed to provide ground simulation capability to the U.S. research community in order to supplement the limited opportunities to access the International Space Station and other platforms for microgravity research. The MSSF is designed to support biological research on microorganisms, cells, tissues, small plants and small animals. The simulator provides NASA with an alternative platform for microgravity research and creates the opportunity to conduct experiments on the space station in parallel with conditions of simulated microgravity on the ground.

Astronaut Donald Thomas conducts the Fertilization and Embryonic Development of Japanese Newt in Space (AstroNewt) experiment at the Aquatic Animal Experiment Unit (AAEU) inside the International Microgravity Laboratory-2 (IML-2) science module. The AstroNewt experiment aims to know the effects of gravity on the early developmental process of fertilized eggs using a unique aquatic animal, the Japanese red-bellied newt. The newt egg is a large single cell at the begirning of development. The Japanese newt mates in spring and autumn. In late autumn, female newts enter hibernation with sperm in their body cavity and in spring lay eggs and fertilized them with the stored sperm. The experiment takes advantage of this feature of the newt. Groups of newts were sent to the Kennedy Space Center and kept in hibernation until the mission. The AAEU cassettes carried four newts aboard the Space Shuttle. Two newts in one cassette are treated by hormone injection on the ground to simulate egg laying. The other two newts are treated on orbit by the crew. The former group started maturization of eggs before launch. The effects of gravity on that early process were differentiated by comparison of the two groups. The IML-2 was the second in a series of Spacelab flights designed to conduct research by the international science community in a microgravity environment. Managed by the Marshall Space Flight Center, the IML-2 was launch on July 8, 1994 aboard the STS-65 Space Shuttle Orbiter Columbia mission.

Astronaut Donald Thomas conducts the Fertilization and Embryonic Development of Japanese Newt in Space (AstroNewt) experiment at the Aquatic Animal Experiment Unit (AAEU) inside the International Microgravity Laboratory-2 (IML-2) science module. The AstroNewt experiment aims to know the effects of gravity on the early developmental process of fertilized eggs using a unique aquatic animal, the Japanese red-bellied newt. The newt egg is a large single cell at the begirning of development. The Japanese newt mates in spring and autumn. In late autumn, female newts enter hibernation with sperm in their body cavity and in spring lay eggs and fertilize them with the stored sperm. The experiment takes advantage of this feature of the newt. Groups of newts were sent to the Kennedy Space Center and kept in hibernation until the mission. The AAEU cassettes carried four newts aboard the Space Shuttle. Two newts in one cassette are treated by hormone injection on the ground to simulate egg laying. The other two newts are treated on orbit by the crew. The former group started maturization of eggs before launch. The effects of gravity on that early process were differentiated by comparison of the two groups. The IML-2 was the second in a series of Spacelab flights designed to conduct research by the international science community in a microgravity environment. Managed by the Marshall Space Flight Center, the IML-2 was launched on July 8, 1994 aboard the STS-65 Space Shuttle mission, Orbiter Columbia.

Dr. Lisa E. Freed of the Massachusetts Institute of Technology and her colleagues have reported that initially disc-like specimens tend to become spherical in space, demonstrating that tissues can grow and differentiate into distinct structures in microgravity. The Mir Increment 3 (Sept. 16, 1996 - Jan. 22, 1997) samples were smaller, more spherical, and mechanically weaker than Earth-grown control samples. These results demonstrate the feasibility of microgravity tissue engineering and may have implications for long human space voyages and for treating musculoskeletal disorders on earth. Final samples from Mir and Earth appeared histologically cartilaginous throughout their entire cross sections (5-8 mm thick), with the exception of fibrous outer capsules. Constructs grown on Earth (A) appeared to have a more organized extracellular matrix with more uniform collagen orientation as compared with constructs grown on Mir (B), but the average collagen fiber diameter was similar in the two groups (22 +- 2 nm) and comparable to that previously reported for developing articular cartilage. Randomly oriented collagen in Mir samples would be consistent with previous reports that microgravity disrupts fibrillogenesis. These are transmission electron micrographs of constructs from Mir (A) and Earth (B) groups at magnifications of x3,500 and x120,000 (Inset). The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Credit: Proceedings of the National Academy of Sciences.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.

NASA’s Marshall Space Flight Center celebrated the 65th anniversary of its founding on July 19, 2025, with a free, public celebration at The Orion Amphitheater in Huntsville, Alabama. Thousands of team members, families, and “Rocket City” residents took part, enjoying live music, games, food, and exhibits commemorating Marshall’s legacy of ingenuity and service to the U.S. space program. During a program for the celebration, guests heard remarks from Joseph Pelfrey, director of NASA Marshall, Huntsville Mayor Tommy Battle, and Kamerra Liles, assistant general manager of The Orion Amphitheater, followed by Pelfrey sharing a new video overview about Marshall. The program continued with a video presentation from NASA astronauts from the Expedition 72 crew – which contributed more than 1,000 total hours of scientific studies on plant growth, stem cell growth for treating diseases, the resilience of microorganisms to the harsh space environment, and more on the International Space Station. NASA astronauts Suni Williams and Don Pettit, who served as space station commander and flight engineer, respectively, shared their experiences in space with the community and told the audience how vital Marshall’s on the ground support was to their mission success. Marshall has shaped and expanded human space exploration every decade since the NASA center opened its doors on July 1, 1960. The center’s civil service and contract workers built the nation’s flagship rockets, from the Saturn V to the SLS (Space Launch System), managed the space shuttle propulsion program, delivered the Chandra X-ray Observatory and critical elements of the International Space Station to orbit, and continue to spearhead numerous advances in science and engineering.